慢性丙型肝炎患者IL-28B基因多态性与中医证型及肝脏生化、APRI的相关性研究

Study on the correlation between the IL-28B gene polymorphism and Traditional Chinese Medicine syndrome,liver biochemical characteristics and APRI in patients with chronic hepatitis C

目的:对慢性丙型病毒性肝炎的IL28B基因SNP进行分型,了解慢性丙型肝炎患者中IL28B不同等位基因的中医证候分布及其与肝脏生化、APRI评分的相关性。方法:采用焦磷酸测序方法对105例慢性丙型病毒性肝炎初治患者的IL28B基因rs12979860 SNP位点的基因型进行检测,完善其肝脏生化、血常规及B超检查,并对其行中医辨证分型,比较不同等位基因的中医证型分布及不同证型的肝脏生化情况、APRI评分。结果:105例慢性丙型肝炎患者的IL28B基因分型中CC型占90例,此分型中中医证型为肝肾阴虚者最多(37例,占41.1%),其AST及APRI评分比其他证型组明显升高,且其发生肝硬化的比例最高(P<0.05)。结论:IL28B等位基因为CC的慢性丙肝患者中中医证型为肝肾阴虚的患者较其他证型患者肝脏生化异常最明显,APRI评分最高,提示肝肾阴虚组患者潜在发生肝硬化的风险更高。

Objective ：To study the correlation between the IL-28B gene polymorphism and Traditional Chinese Medicine syndrome, liver biochemical characteristics and APRI in patients with chronic hepatitis C. Mcthods：Pyrosequencing method was used to test genotype IL28B gene rs12979860 SNP and completed the liver biochemistry test, blood routine test , ultrasound examinatio in 105 cases of naive chronic HCVpatients visited our hospital. Then the TCM syndrome types were classified and the distribution of TCM Syndromes and their correlation with biochemical characteristics and APRI score were compared. Results：There were 90 cases CC type of IL28B gene in 105 chronic HCV patients visited our hospital. ,the liver and kidney yin deficiency was the most common TCM syndrome in these patients（37 cases, 41.1% ）, AST and APRI score in this TCM syndrome were significantly higher than other syndromes, and the percentage of liver cirrhosis was the high- est in this syndrome. （P 〈 0.05 ）. Conclusion ：The liver biochemical abnormalities and APRI score are most obvious in the liver and kidney Yin deficiency syndrome of IL28B allele for CC in patients with chroinc HCV , so the patients in liver and kidney Yin deficiency syndrome has the higher potential risk to cirrhosis.