组合应用输血相容性检测技术的研究

Study on the combination of compatibility testing method

目的分析不同交叉配血试验技术在保障安全输血中的重要作用,探索疑难交叉配血检测策略。方法同步应用盐水介质、聚凝胺法、柱凝集法及试管抗球蛋白法交叉配血试验技术进行了2 199例ABO与RhD同型血液相容性检测,并对检测结果进行统计分析。结果盐水介质、聚凝胺法、柱凝集法及试管抗球蛋白法交叉配血阳性试验结果分别为98、944、1 304及1 252例。98例盐水介质交叉配血阳性中而聚凝胺法、柱凝集法及试管抗球蛋白法交叉配血阳性检出率分别为76.53%、88.78%及73.47%。柱凝集法与试管抗人球法交叉配血结果无统计学差异(P>0.05)。结论盐水介质交叉配血技术在血液相容性检测中必不可少。应用多种交叉配血技术综合分析疑难交叉配血结果,能够进一步保证临床输血安全。

Objective To analyze the important function of different crossmatching technologies on the saiety of blood transfusion,and to study the test strategy to approach difficult clinical blood matching. Methods 2 199 tests were performed by the crossmatch methods of Immediate-spin （IS）, polybrene, column agglutination technology （CAT） and indirect anti- globulin test（IAT）at the same time. Afterwards, the tests were analyzed. Results The positive cases of IS, polybrene, CAT and IAT were 98, 944, 1 304 and 1 252. In the 98 positive cases of IS, while the positive rates of polybrene, CAT and IAT were 76. 53%,88. 78% and 73.47%. There were no significant differences in CAT and IAT （P〉0. 05） i Conclusion Imme- diate-spin technology is necessary in blood compatibility tests. Application of different crossmatching technologies can help analyze the complicated samples from difficult crossmatching and further ensure the safety of clinical blood transfusion.