高效液相色谱法同时检测植物甾醇与植物甾醇酯

Simultaneous Detection of Phytosterols and Phytosterol Esters with HPLC

采用反相高效液相色谱,对同时检测植物甾醇和甾醇酯的分析方法进行了研究。通过检测条件优化,植物甾醇和甾醇酯的12种不同组分在以丙酮和乙腈(3∶1)为流动相,柱温30℃,流动相流速1.0mL/min,等度洗脱的条件下,达到最好分离效果。此方法不需要样品的前处理,分析时间25 min,检出限为5.1~1.1μg/mL,定量限为17.0~137.0μg/mL,回收率93.81%~111.98%。植物甾醇与甾醇乙酸酯在0.01~1.0 mg/mL的浓度范围内呈线性相关,甾醇亚油酸酯与甾醇油酸酯在0.1~1.0 mg/mL范围内具有良好的线性关系,标准曲线相关系数均超过0.998。该方法可应用于多种甾醇和甾醇酯产品的同时检测。

Simultaneous detection method of phytosterols and phytosterol esters was studied by reversed - phase HPLC. Acetone and acetonitrile （3：1 ）was used as mobile phase, 12 compounds of phytosterols and phytosterol esters could be separated completely under the conditions of flow rate i. 0 mL/min, column temperature 30 ℃ and isocratic elution. Analysis time was only 25 minutes in this method, and there was no sample pretreatment. Limit of detection ranged from 5.1 to 41. 1 μg/mL, and limit of quantitative ranged from 17. 0 to 137. 0 μg/mL, and recovery rates ranged from 93.81% to 111.98%. Sterol linoleate and sterol oleate in the concentration range of 0. 01 -1.0 mg/mL showed a linear correlation. Linearity range of sterol linoleate and sterol oleate was 0. 1 - 1.0 mg/mL. All the regression coefficients of standard curves were over 0. 998. This method could be used in simultaneous detection of different sterol and sterol ester products, and worthy of promotion.