糖尿病周围神经病变患者外周血淋巴细胞蛋白激酶C活性与胰岛素抵抗的检测及意义

The Detection and Meaning of the Active and Insulin Resistance of Peripheral Neuropathy Patients Peripheral Neuropathy

目的探讨伴有周围神经病变的糖尿病患者外周血淋巴细胞蛋白激酶C的活性与胰岛素抵抗的关系。方法选取有胰岛素抵抗且合并不同程度周围神经病变的糖尿病患者40例作为实验组;取40名健康人为对照1组;对照2组为40例患者无周围神经病变,但具有胰岛素抵抗的糖尿病患者。分别抽取外周血,检测各组外周血中淋巴细胞蛋白激酶C的活性,各分组间进行对比。结果具有周围神经病变的胰岛素抵抗患者,无论是淋巴细胞胞膜PKC的活性还是胞质PKC活性与对照1、2组比较,均有明显增高,且差异具有统计学意义(P<0.05);单纯胰岛素抵抗组较健康组比较,无论是淋巴细胞胞膜PKC的活性还是胞质PKC活性,均明显增高,差异有统计学意义(P<0.05)。结论外周血淋巴细胞蛋白激酶C活性与胰岛素抵抗密切相关,对胰岛素抵抗的诊断有指导意义。

Objective To investigate the changes of protein kinase C（PKC）activity in Diabetic peripheral neuropathy（DNP）in patients with insulin resistance（IR）.Methods PKC obtained from the cytoplasm and cellular membrane of PBLs in patients with Diabetic peripheral neuropathy and normal controls was isolated and purified for activity assaying by isotopeγ-P-ATP catalytic activity test.Results Patients with Diabetic peripheral neuropathy and IR were associated with markedly higher PKC activity in1 ymphocytes as well as higher cellular membrane PKC activity and cytoplasm PKC activity.ConclusionWhen compared with normal controls.Patients with Diabetic peripheral neuropathy and IR are featured by considerably increased activity and the level of activation of PKC in PBL.