红细胞悬液中致热源性细胞因子对非溶血性发热性输血反应发生的影响

Influence of inflammatory cytokines in febrile non-hemolytic transfusion reaction

目的检测储存前滤除白细胞对红细胞悬液中致热源性细胞因子含量的影响,以及致热源性细胞因子与非溶血性发热性输血反应(FNHTR)的关系,以期得到较为全面的认识。方法随机抽取普通红细胞悬液及去除白细胞的红细胞悬液2种成分;每种成分按照自制备到输注前的储存时间分组;用ELISA方法检测致热源性细胞因子含量,观察输血反应情况。结果 3种致热源性细胞因子含量在检测期内的不同时间点略有升高,与储存时间无平行关系。2例发生发热性输血反应病例中致热源性细胞因子含量均非最高值。结论红细胞悬液因为储存在较低温度下,所以在储存过程中致热源性细胞因子释放量较小,不足以引起非溶血性发热反应。

Objective To investigate the influence of filtering leukocyte before storage on inflammatory cytokines content in e- rythroeyte suspension, and to explore its correlation with febrile non -hemolytic transfusion reactions （RNHTR） , in order to get a comprehensive understanding. Methods Randomly choose some filtered and unfiltered erythrocyte suspension; each compo- nent was grouped according to the storage time between preparation and infusion ; to detect the contents of proinflammatory cyto- kines with ELISA, and the blood transfusion reaction was observed. Results The contents of 3 proinflammatory cytokines in- creased at different time points and did not have parallel relationship with time. The two cases of FNHTR did not have the high- est content of proinflammatory cytokines. Conclusion Because red cell suspension is stored at a lower temperature, the release of inflammatory cytokines during storage is small and not enough to cause FNHTR.