泽泻汤对油酸诱导HepG2细胞脂肪堆积的抑制作用

Inhibition of Zexie Decoction on oleic acid-induced fat accumulation in HepG2 cells

目的评价泽泻汤95%乙醇、50%乙醇和水提取物抑制HepG2肝细胞脂肪累积能力。方法采用油酸诱导HepG2细胞脂肪累积建立模型,以噻唑兰染色和油红O染色法吸光度值为指标,结合细胞内脂滴形态,评价泽泻汤对油酸诱导HepG2细胞脂肪累积的干预作用。结果泽泻汤提取物作用HepG2细胞后,经染色后均发现细胞内脂滴明显减少,且质量浓度越高脂类含量越少,50%乙醇提取物和水提物在终质量浓度160、320μg/m L时,细胞内脂滴与模型组相比明显降低(P<0.01),95%乙醇提物80μg/m L作用细胞24 h后,细胞内脂滴较模型组有减少(P<0.05)。结论 3种泽泻汤提取物均能够减少细胞内脂肪的堆积,其中以50%乙醇提取物对HepG2细胞脂肪堆积抑制能力最强。

Objective To evaluate the effects of 95% ethanol, 50% ethanol, and distilled water extracts from Zexie Decoction on fat accumulation of HepG2 hepatic cells. Methods Fat accumulation model in HepG2 cells was induced by oleic acid. Absorptions of MTT method and oil red O method and intracellular lipid droplet morphology were used to evaluate the effect of Zexie Decoction on oleic acid-induced fat accumulation in HepG2 cells. Results The lipid droplet of HepG2 cells was significantly reduced after cultivation with Zexie Decoction extracts, and the higher concentration, the less lipid contents. After culturing with 50% ethanol extract（160 μg/mL） and water extract（320 μg/mL）, the lipid droplet of HepG2 cells reduced significantly compared with model group（P〈0.01）, and after culturing with 95% ethanol extract with the maximum concentration of 80 μg/mL for 24 h. the lipid droplet of HepG2 cells was also reduced（P〈0.05）. Conclusion Three extracts of Zexie Decoction show the effect of lipid-decreasing, and 50% ethanol extract is the best lipid-lowering ingredient.