慢性丙型肝炎患者外周血单个核细胞中干扰素刺激基因及相关信号通路基因表达的研究

Study on Expression Interferon Stimulating Gene and Related Signal Pathway Gene in Peripheral Blood Mononuclear Cells of Patients with Chronic Hepatitis C

目的观察慢性丙型肝炎(chronic hepatitis C,CHC)患者外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中干扰素刺激基因(stimulator of interferon genes,STING)、维甲酸诱导基因Ⅰ(retinoic acid inducible gene 1,RIG-1),以及Ⅰ型干扰素α(Interferon-α,IFN-α)和β(IFN-β)mRNA的表达,为治疗CHC提供新思路。方法选择2016年1月~2017年1月在武汉大学人民医院感染科未经治疗的CHC患者113例,以及同期进行体检的健康人94例,通过实时荧光定量PCR检测STING,RIG-1样受体,以及IFN-α和IFN-βmRNA表达,用2^(-ΔΔCt)的方法计算相对表达量,并对计算结果进行统计学分析。结果 CHC患者外周血中STING,RIG-1,IFN-α和IFN-βmRNA的表达分别是健康对照组的0.018,0.361,0.578和0.573倍,两组间的比较差异均有统计学意义(t=8.28,4.26,2.18和2.07,均P<0.05);健康人STING与IFN-αmRNA,IFN-βmRNA表达呈正相关性(r=0.487,0.207,均P<0.05);CHC患者STING与IFN-αmRNA,IFN-βmRNA表达无相关性(r=0.174,0.091,均P<0.05);健康人STING与RIG-1 mRNA表达呈低正相关性(r=0.222,P<0.05),CHC患者STING与RIG-1 mRNA表达无相关性(r=-0.029,P>0.05)。结论与健康人相比,CHC患者STING,RIG-1,IFN-α和IFN-βmRNA表达均降低,且在健康人中STING基因与RIG-1及干扰素基因存在正相关性,而CHC中无相关性。

Objective To detect mRNA expressions of STING and type I interferons（IFN-α and IFN β） in peripheral blood mononuclear cells （PBMCs） of patients with chronic hepatitis C（CHC）. Methods 113 patients with CHC and 94 healthy controls were collected from Renmin Hospital of Wuhan University during January 2015 and January 2016. Expressions of mRNA of STING, RIG-1 ,IFN-α and IFN-β were detected by real-time quantitative PCR, and their relative expression values were obtained by 2^△△Ct, method and the results were statistically analyzed. Results The expression of mRNA of STING, RIG-1 ,IFN-α and IFN-β in CHC were 0. 018,0. 361,0. 578 and 0. 573 times than its in healthy controls respectively and the differences were statistically significant （t = 8.28,4.26,2.18 and 2.07, all P〈 0.05）. Pearson correlation analysis showed that mRNA expressions of STING in healthy controls were positively correlated with that of IFN-γ mRNA and IFN -13 mR- NA （r=0. 487 and 0. 207 ,all P〈0.05） ,which had no correlation in CHC （r：0. 174 and 0. 091 ,all P〈0.05）. The expres sions of STING mRNA was positively correlated with that of RIG 1 mRNA in heahhycontrols （r=0. 222,P〉0.05） ,but there was no correlation of expression in CHC between STING mRNA and RIG-1 mRNA （r= -0. 029 ,P;〉0. 05）. Conclusion Compared with healthy controls, the expression of STING, RIG-1, IFN-α and IFN-β mRNA were all reduced. There was positive correlation between STING and RIG-1,IFN-α and IFN-β in healthy controls, but no correlation in CHC.