广西地区壮族和汉族冠心病患者胱抑素C水平及其基因多态性的比较研究

Comparative Study of Cystatin C and Its Gene Polymorphism of Patients with Coronary Heart Disease between Zhuang and Han in Guangxi Region

目的探讨广西地区壮族冠心病患者血清中胱抑素C(Cys C)水平及其基因位点+148 G/A和-82 G/C的多态性与同地区汉族冠心病(CHD)患者及两个民族正常人的不同。方法采用免疫比浊法检测壮族、汉族CHD患者和壮族、汉族正常个体(各100例)血清中Cys C水平,聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测Cys C基因位点+148和-82的多态性,并分析上述数据和临床资料。结果①两组CHD组血清中Cys C水平和临床资料与两组正常组间的差异有统计学意义(P值均<0.05);②四组中Cys C基因位点+148和-82等位基因分布频率符合H-W群体遗传平衡法则(P值均>0.05),具有群体代表性。CHD组Cys C基因+148和-82位点不同基因型分布频率较同民族正常组虽有差异,但差异均无统计学意义(X^2=0.760~2.090,P>0.05);③CHD病组血清中Csy C水平与Cr呈正相关(r=0.597,P<0.001)。结论 Cys C基因位点+148和-82的多态性与广西地区的壮族、汉族CHD相关性需作进一步研究,但肾损伤引起的高Cys C血清水平可能是广西地区壮族、汉族CHD患者的一个危险因素。

Objective To investigate the difference of serum levels of cystatin C and its gene polymorphism of patients with CHD and normal people between Zhuang and Han in Guanxi region. Methods The levels of serum cystatin C in Zhuang CHD patients, Han CHD patients, Zhuang normal people and Han normal people（each of 100 cases）were detected by Immu noturbidimetric Assays,Cys C＋ 148 and Cys C-82 genotypes were conducted by using PCR-RFLP,and that data and clinical data were analyzed. Results The difference of Cys C levels and clinical data between two CHD groups and two normal groups were statically significant （all P〈0.05）. ①The Cys C levels of two CHD groups was not statically significant difference （P=0. 156）. ②The CysC＋148 and Cys C-82 of 4 groups conform the Hardy-Weinberg population genetic equilibrium law （all P〉0.05）. The genotypes frequency difference of Cys C＋148 and Cys C-82 between two CHD groups and two normal group were no statically significant （Z2 =0. 760~2. 090 ,P〉0. 05）. ③The serum Cys C level and Cr of CHD group were positively correlated （r = 0. 597, P 〈 0. 001 ）. Conclusion The correlation Cys C ＋ 148,-82 polymorphism and Guangxi Zhuang CHD were in need of further study,but the kidney damage caused by the high serum Cys C level may be a risk factor for Guangxi Zhuang and Han CHD patients.