HPLC法同时测定大血藤中原儿茶酸、红景天苷和绿原酸含量

Simultaneous determination of protocatechuic acid, salidroside, and ehlorogenie aeidin sargentodoxa cuneata by HPLC

目的建立同时测定大血藤中原儿茶酸、红景天苷和绿原酸含量的HPLC法。方法色谱柱为WatersXSELECTCSHC18（150mm×4．6mm，5gm），以乙腈-甲醇-0．2％磷酸水为流动相进行梯度洗脱，体积流量0．8ml／min，检测波长260nm，柱温35℃。结果原儿茶酸、红景天苷和绿原酸分别在0．0020～0．0120、0．060O～O．3602和0．0750～4．5006mg／ml内呈良好线性关系，平均加样回收率分别为98．01％、98．53％、101．10％，RSD分别为0．07％、0．12％、0．92％。结论该方法操作简便、准确、重复性好，可为大血藤质量控制提供依据。

Objective To establish a method for the determination ofprotocatechuic acid, salidroside, and chlorogenic acid in Sargentodoxa cuneata. Methods The separation was performed on a Waters XSELECT CSH C18 （150 mm × 4.6 mm, 5μm） with methanol-acetonitrile-0,2 % phosphoric acid as the mobile phase in a gradient elution at a flow rate of 0.8 ml/min. The detection wavelength was 260 nm and the column temperature was 35℃. Results The linear ranges of protocatechuic acid, salidroside, and chlorogenic acid were 0.002 0-0.012 0, 0.060 0-0.360 2, 0.075 0-4.500 6 mg/ml, respectively. The average recoveries were 98.01% （RSD=-0.07%）, 98.53 % （RSD=0.12%）, and 101.10 % （RSD=l.92%）, respectively. Conclusions The method is simple, accurate, and highly reproducible, which could provide the scientific evidence for the quality control of Sargentodoxa cuneata,