摘要
目的建立同时测定大血藤中原儿茶酸、红景天苷和绿原酸含量的HPLC法。方法色谱柱为WatersXSELECTCSHC18(150mm×4.6mm,5gm),以乙腈-甲醇-0.2%磷酸水为流动相进行梯度洗脱,体积流量0.8ml/min,检测波长260nm,柱温35℃。结果原儿茶酸、红景天苷和绿原酸分别在0.0020~0.0120、0.060O~O.3602和0.0750~4.5006mg/ml内呈良好线性关系,平均加样回收率分别为98.01%、98.53%、101.10%,RSD分别为0.07%、0.12%、0.92%。结论该方法操作简便、准确、重复性好,可为大血藤质量控制提供依据。
Objective To establish a method for the determination ofprotocatechuic acid, salidroside, and chlorogenic acid in Sargentodoxa cuneata. Methods The separation was performed on a Waters XSELECT CSH C18 (150 mm × 4.6 mm, 5μm) with methanol-acetonitrile-0,2 % phosphoric acid as the mobile phase in a gradient elution at a flow rate of 0.8 ml/min. The detection wavelength was 260 nm and the column temperature was 35℃. Results The linear ranges of protocatechuic acid, salidroside, and chlorogenic acid were 0.002 0-0.012 0, 0.060 0-0.360 2, 0.075 0-4.500 6 mg/ml, respectively. The average recoveries were 98.01% (RSD=-0.07%), 98.53 % (RSD=0.12%), and 101.10 % (RSD=l.92%), respectively. Conclusions The method is simple, accurate, and highly reproducible, which could provide the scientific evidence for the quality control of Sargentodoxa cuneata,
出处
《国际中医中药杂志》
2017年第6期535-538,共4页
International Journal of Traditional Chinese Medicine
基金
上海市科学技术委员会资助项目(13401901400)
关键词
大血藤
原儿茶酸
红景天苷
绿原酸
色谱法
高压液相
Sargentodoxa cuneata
Protocatechuic acid
Rhodioloside
Chlorogenic acid
Chromatography, high pressure liquid