miRNA-31在结直肠癌细胞中的表达及其作用机制的研究

Expression of miRNA-31 in colorectal cancer and its action mechanism

目的探讨miRNA-31在结肠癌细胞生长及转移过程中的作用,为结直肠癌发生、转移机理提供实验依据。方法采用miRNA检测芯片分析10例结直肠癌患者癌组织和癌旁正常组织的miRNA表达谱;RT-PCR检测结直肠癌细胞系与正常结肠细胞miRNA的差异表达;用RT-PCR和免疫印迹等方法进行miRNA-31生物学功能验证;观察结直肠癌细胞系中miRNA-31及其调控靶基因对细胞增殖、周期和侵袭能力的调控作用。结果与癌旁对照组织相比,miRNA-31在结直肠癌组织中高效表达,并与另外39例患者结直肠癌组织标本检测一致,同时miRNA-31的表达水平与结直肠癌患者肿瘤组织的转移性和TNM分期有关。miRNA-31可促进结直肠癌细胞系HCT-116细胞的增殖和侵袭。结论 miRNA-31可作为结直肠癌发生发展的潜在分子标志物,为结直肠癌的临床检测提供新的检测靶点。

Objective To investigate the role of miRNA-31 in the growth and metastasis of colon cancer cells, and to provide experimental evidence for the mechanism of carcinogenesis and metastasis of colorectal cancer. Methods The expression of miRNA in 10 cases of colorectal cancer tissues and adjacent normal tissues were detected by miRNA chip, and the differential expression of colorectal cancer cell line and normal colon cell line miRNA was determined by RT-PCR. Then, we further testified the miRNA expression in other thirty-nine CRC tissues and several colorectal cancer cell lines by RT-PCR. The relationship between CRC stage and miRNA-31 expression level were also measured. RT-PCR and immunoblotting were used to verify the biological function of miRNA-31. The regulatory role of miRNA-31 and its target genes on cell proliferation, cell cycle and invasion in colorectal cancer cell lines was investigated. Results miRNA-31 was highly expressed in colorectal cancer tissues compared with adjacent control tissues, and was consistent with the other39 patients with colorectal cancer. Meanwhile, miRNA-31 expression was closely related to the tumor metastasis and TNM stage. Conclusion Mi RNA-31 can be used as a potential molecular marker for the development of colorectal cancer, and provide a new target for clinical detection of colorectal cancer.