摘要
利用隐马可链夫模型(HMM)对芝麻全基因组Hsp70基因进行了系统分析,共鉴定出21个Hsp70基因,其中17个属于Dnak基因亚家族,4个属于Hsp110/SSE基因亚家族。根据系统进化树分析和亚细胞定位结果,将芝麻21个Hsp70基因分为6个亚组,其中I亚组3个,定位于叶绿体;II亚组2个,定位于线粒体;III亚组5个,定位于内质网;IV亚组6个,定位于细胞质基质;V亚组4个,属于Hsp110/SSE基因家族;VI亚组1个。芝麻Hsp70基因启动子区域顺式作用元件功能预测结果显示,Hsp70基因参与芝麻的生长发育和逆境胁迫响应等生理过程。
In this study, Hsp70 genes in the sesame genome were systematically analyzed with Hidden Markov Model （ HMM） . A total of 21 SiHsp70 genes were identified, among which, seventeen belong to DnaK subfamily and four of them belong to Hsp110/SSE subfamily. Based on the phylogenetic and subcellular localization analysis, the 21 SiHsp70 genes were classified into 6 subgroups. Three chloroplast-localized genes belong to subgroup I, two mitochondrial-localized genes belong to subgroup II, five ER-localized genes belong to subgroup III, six cytoplasmic-localized genes belong to subgroup IV, four Hsp110/SSE-type genes belong to subgroup V, and one gene belongs to subgroup VI. The cis-acting elements in the promoters of SiHsp70 genes were further analyzed, and the results demonstrated that they were involved in growth, de-velopment and stress response.
出处
《江苏农业学报》
CSCD
北大核心
2017年第3期493-502,共10页
Jiangsu Journal of Agricultural Sciences
基金
江苏省农业科技自主创新基金项目[CX(14)2038]
关键词
芝麻
HSP70基因
生物信息学分析
Sesamum indicum
Hsp70 gene
identification
bioinformatics analysis
