抗茄链格孢菌放线菌的筛选及鉴定

Screening and identification of antagonistic actinomyces against Alternaria solani

以茄链格孢菌为靶标菌,采用平板对峙法和生长速率法对分离自药用植物的221株放线菌进行筛选,通过生理生化特征、培养特征、形态特征和基于16S rRNA基因序列的相似性分析对活性菌株的种属进行了鉴定。结果表明,抑菌率75%以上放线菌有3株,其中菌株3150被鉴定为环圈链霉菌(Streptomyces anulatus)的一个菌株,菌株3679被鉴定为氢化链霉菌(Streptomyces hydrogenans)的一个菌株,菌株4536被鉴定为链霉菌属(Streptomyces sp.)菌株。薄层色谱分析结果显示菌株3679代谢产物丰富且分离度好,进一步分离可望得到具有较好生防作用的化合物。

To identify new bio-control agents against Alternaria solani, 221 strains of actinomyces from different me-dicinal plants were screened by plate confrontation method and growth rate method. The active strains were identified ac-cording to the physiological, cultural and morphological characteristics and 16S rRNA gene sequence analysis. There were 3 strains with inhibition rate against A. solani more than 75%, among which strain 3150 was identified as Streptomyces anula-tus, strain 3679 was identified as S. hydrogenans, and strain 4536 was identified as the genus Streptomyces. Thin layer chro-matography showed that strain 3679 was rich in metabolites and high in resolution. The compounds with bio-control effect was expected after further separation.