2株降解菌对土壤中二甲戊灵的降解效果

Degradation effect of two bacterial strains on soil pendimethalin

在实验室条件下,研究了细菌菌株JY-2和JY-5对二甲戊灵污染土壤的修复作用及其影响因素,并采用生物法进行验证。结果表明,二甲戊灵的降解率与接种量呈正相关,其中,当接种量为10%(0.6×109CFU/g)时,接种后15 d菌株JY-2和JY-5对土壤中200 mg/kg二甲戊灵的降解率分别达到66.18%和65.66%,低于此接种量时降解菌的降解作用较弱;土壤温度及含水量对菌株降解作用具有显著影响,最适土壤温度为30℃,最佳含水量为600 g/kg,在此条件下,菌株JY-2和JY-5对二甲戊灵的降解率分别为73.25%和67.07%;接种降解菌JY-2和JY-5可不同程度降解土壤中二甲戊灵,减轻二甲戊灵对玉米和棉花生长的抑制作用,具有一定的土壤修复作用。

JY-2 and JY-5, 2 strains of degrading bacterium, were taken as the tested strains to study the influencing factors of bioremediation of soil contaminated by pendimethalin, and the degradation effect was verified by biological meth-od. A positive correlation was shown between the degradation rate and the inoculum size. The degradation rates of the 200 mg/kg pendimethalin by JY-2 and JY-5 at 0.6＆#215;109 CFU/g 15 d post-inoculation were 66.18% and 65.66% respectively. The soil temperature and moisture affected the degradation obviously. Soil temperature at 30 ℃ and soil moisture of 600 g/kg generated the highest degradation rates of JY-2 and JY-5, 73.25% and 67.07%, respectively. Inoculation of JY-2 and JY-5 mitigated the growth inhibition of maize and cotton in soil contaminated by pendimethalin, indicative of a certain de-gree of soil remediation.