丝氨酸蛋白酶编码基因prtP对副干酪乳杆菌黏附特性的影响

Adhesion characteristics of Lactobacillus paracasei influenced by serine protease encoding gene prtP

为探讨丝氨酸蛋白酶编码基因prtP对副干酪乳杆菌黏附特性的影响,利用λRed重组系统敲除副干酪乳杆菌的prtP基因,构建副干酪乳杆菌prtP基因缺失突变株,比较突变株与亲本株的表面结构、黏附特性的差异。结果表明,prtP基因缺失突变株的生长速度变慢,疏水性和自聚合性明显下降,抑菌效果无显著性差异,对抗生素的敏感性发生了显著变化,体外对细胞的黏附性显著降低。可见,prtP基因对副干酪乳杆菌的黏附特性十分重要,该基因缺失将改变菌株的生物学特性。

To investigate the effects of serine protease encoding gene prtP on the adhesion of Lactobacillus paraca-sei, an isogenic prtP mutant was constructed by knocking out prtP gene using λRed recombination system. The surface structure and adhesion characteristics of the mutant were studied. The prtP gene mutant strain showed slower growth rate, decreased hydrophobicity and self condensing ability. The sensitivity to antibiotics of the mutant strain changed in compari-son to parental strain, and the adhesion of cells dropped significantly in vitro. In conclusion, the prtP gene is important for the adhesion of L. paracasei, and the deletion could change the biological characteristics of the strain.