SPA-PEI交联物及基于交联物构建的抗体靶向性核酸转移系统对培养细胞的毒性作用

Cytotoxicities of SPA-PEI conjugate and it's antibody-targeted non-virus gene delivery system to cultured cells

目的:评价新型抗体靶向性核酸转移系统及其核心组份[葡萄球菌A蛋白-聚乙烯亚胺交联物(SPAPEI交联物)]对培养细胞的毒性作用。方法:将SPA-PEI交联物与转铁蛋白受体的抗体和绿色荧光蛋白质粒以适宜的质量比混合,组装成一种抗体靶向性核酸转移系统;采用不同浓度SPA-PEI交联物或抗体靶向系统分别处理Hep G2人肝癌细胞不同时间后MTT比色法测定细胞的存活率。结果:SPA-PEI交联物对培养的Hep G2人肝癌毒性作用有明显的浓度依赖性和时间依赖性,但其杀伤效应较PEI降低;抗体靶向系统的细胞毒作用较弱,其作用细胞的存活曲线近似于SPA。结论:SPA-PEI交联物对培养细胞有较强的杀伤效应,但以其为核心组份构建的抗体靶向性核酸转移系统无明显细胞毒性作用。

Objective： To evaluate cytotoxicities of the antibody-targeted non-virus gene delivery system and it＇s key component[ staphylococcal protein A （ SPA ） - polyethylenimine （ PEI ） conjugate （ SPA- PEI conjugate ） ] to the cultured cells. Methods： One kind of antibody-targeted non-virus gene delivery system was prepared by mixing SPA-PEI conjugate, the enhanced green fluorescent protein plasmid （pEGFP-C1 ） and the transferrin receptor- specific antibody at room temperature for 60 minutes based on their optimal mass ratio. Following incubation with various concentration of the SPA-PEI conjugate or the gene delivery system for different time interval, the cell survivals of cultured HepG2 cells were assayed by MTT colormetric method. Results： SPA-PEI conjugate showed remarkable cytotoxicity to the cells in manner of concentration-dependence and time-dependence, but killing effect was reduce compared with free PEI. However, the antibody-targeted gene delivery system exerted few killing effect on cultured cells,with similar survival curve to free SPA. Conclusion： SPA-PEI conjugate has obvious cytotoxicity to cultured cells, however its antibody-targeted non-virus gene delivery system displays few killing effect.