洋葱总黄酮处理后糖尿病模型大鼠视网膜组织中氧化应激、细胞凋亡程度的研究和分析

Study and analysis of oxidative stress and cell apoptosis in retinal tissue of diabetic model rats after total flavonoids of onion treatment

目的:研究洋葱总黄酮处理后对糖尿病模型大鼠视网膜组织中氧化应激、细胞凋亡程度的影响。方法:选择雄性SD大鼠作为实验动物并随机分为对照组、糖尿病组和总黄酮低剂量组(总黄酮L组)、总黄酮高剂量组(总黄酮H组),腹腔注射链脲佐菌素以建立糖尿病模型,总黄酮L组和H组分别给予100mg/kg和200mg/kg的洋葱总黄酮灌胃干预。干预8周后,收集视网膜组织并测定氧化应激反应程度、细胞凋亡程度。结果:糖尿病组视网膜组织中P2X7、ROS、-OH、O_2~﹣、H_2O_2、FADD、p-JNK、caspase-8、caspase-3的含量显著高于对照组,Mn-SOD、CAT、GSH、AsA、NGF、BDNF、CNTF的含量显著低于对照组;总黄酮L组和总黄酮H组视网膜组织中P2X7、ROS、-OH、O_2~﹣、H_2O_2、FADD、pJNK、caspase-8、caspase-3的含量显著低于糖尿病组,Mn-SOD、CAT、GSH、AsA、NGF、BDNF、CNTF的含量显著高于糖尿病组;总黄酮H组视网膜组织中P2X7、ROS、-OH、O_2~﹣、H_2O_2、FADD、p-JNK、caspase-8、caspase-3的含量显著低于总黄酮L组,Mn-SOD、CAT、GSH、AsA、NGF、BDNF、CNTF的含量显著高于总黄酮L组。结论:洋葱总黄酮能够抑制糖尿病模型大鼠视网膜组织中的氧化应激和细胞凋亡。

Objective：To study the effect of total flavonoids of onion treatment on oxidative stress and cell apoptosis in the retinal tissue of diabetic model rats.Methods：Male SD rats were selected as experimental animals and randomly divided into control group,diabetes group,low-dosage total flavonoids group（total flavonoids L group）and high-dose total flavonoids（total flavonoids H group）,intraperitoneal injection of streptozotocin was adopted to make diabetes model,and the total flavonoids L group and H group received lavage of 100mg/kg and 200mg/kg total flavonoids of onion for intervention.After 8weeks of intervention,retinal tissue was collected and the degree of oxidative stress and cell apoptosis were determined.Results：P2X7,ROS,-OH,O2^﹣H2O2,FADD,p-JNK,caspase-8and caspase-3levels in retinal tissue of diabetes group were significantly higher than those of control group while Mn-SOD,CAT,GSH,AsA,NGF,BDNF and CNTF levels were significantly lower than those of control group;P2X7,ROS,-OH,O2^﹣H2O2,FADD,p-JNK,caspase-8and caspase-3levels in retinal tissue of total flavonoids L group and total flavonoids H group were significantly lower than those of diabetes group while Mn-SOD,CAT,GSH,AsA,NGF,BDNF and CNTF levels were significantly higher than those of diabetes group;P2X7,ROS,-OH,O2^﹣H2O2,FADD,p-JNK,caspase-8and caspase-3levels in retinal tissue of total flavonoids H group were significantly lower than those of total flavonoids L group while Mn-SOD,CAT,GSH,AsA,NGF,BDNF and CNTF levels were significantly higher than those of total flavonoids L group.Conclusion：Total flavonoids of onion have inhibiting effect on the oxidative stress and cell apoptosis in retinal tissue of diabetic model rats.