急性冠脉综合征患者体内NLRP3多态性与炎性小体活性、内皮损伤程度的相关性

Correlation of NLRP3 polymorphism with inflammasome activity and endothelial damage in patients with acute coronary syndrome

目的:研究急性冠脉综合征患者体内Nod样受体蛋白3(NLRP3)多态性与炎性小体活性、内皮损伤程度的相关性。方法:选择急性冠脉综合征以及稳定型心绞痛的患者,分别作为ACS组和SAP组,另取同期体检的健康志愿者作为对照组。采集外周血并检测NLRP3基因rs10754558位点的多态性,分离血清并测定炎性小体活性指标、内皮损伤指标。结果:ACS组NLRP3基因GG基因型和GC基因型的构成比均显著高于SAP组和对照组,CC基因型的构成比显著低于SAP组和对照组,血清中IL-1β、IL-18、E-selectin、vWF、ET-1的含量显著高于SAP组和对照组,NO的含量显著低于SAP组和对照组;GG基因型、GC基因型ACS患者血清中IL-1β、IL-18、E-selectin、vWF、ET-1的含量显著高于CC基因型,NO的含量显著低于CC基因型,而GG基因型ACS患者血清中IL-1β、IL-18、E-selectin、vWF、ET-1的含量显著高于GC基因型,NO的含量显著低于GC基因型。结论:ACS患者体内NLRP3基因rs10754558位点等位基因G增多会增加炎性小体活性、加重内皮功能损伤。

Objective：To study the correlation of Nod-like receptor protein 3（NLRP3）polymorphism with inflammasome activity and endothelial damage in patients with acute coronary syndrome.Methods：Patients diagnosed with acute coronary syndrome and stable angina pectoris in Mianyang Central Hospital between May 2013 and August 2016 were selected and included in ACS group and SAP group respectively,and healthy volunteers who received physical examination during the same period were selected as control group.Peripheral blood was collected to detect NLRP3 gene rs10754558loci polymorphism,and serum was separated to determine inflammasome activity indexes and endothelial injury indexes.Results：NLRP3gene GG genotype and GC genotype constituent ratio of ACS group were significantly higher than those of SAP group and control group while CC genotype constituent ratio was significantly lower than that of SAP group and control group,and serum IL-1β,IL-18,E-selectin,vWF and ET-1levels were significantly higher than those of SAP group and control group while serum NO level was significantly lower than that of SAP group and control group;serum IL-1β,IL-18,E-selectin,vWF and ET-1levels in ACS patients with GG genotype and GC genotype were significantly higher than those in patients with CC genotype while NO levels were significantly lower than that in patients with CC genotype,and serum IL-1β,IL-18,E-selectin,vWF and ET-1levels in ACS patients with GG genotype were significantly higher than those in patients with GC genotype while NO level was significantly lower than that in patients with GC genotype.Conclusions：The increased NLRP3 gene rs10754558loci alleles G in patients with ACS will increase inflammasome activity and endothelial injury.