宫颈癌组织中miR-100表达量与顺铂耐药的关系研究

Relationship between miR-100 expression in cervical cancer tissue and cisplatin resistance

目的:研究宫颈癌组织中miR-100表达量与顺铂耐药的关系。方法:收集在本院接受顺铂化疗的107例宫颈癌组织作为临床样本,根据化疗效果分为化疗有效的顺铂敏感宫颈癌组织和化疗无效的顺铂敏感宫颈癌组织。检测miR-100的表达量及耐药相关基因、细胞周期相关基因及细胞侵袭相关基因的表达量。结果:顺铂耐药的宫颈癌组织中miR-100的表达量显著低于顺铂敏感的宫颈癌组织,Nek2、P-gp、GST-π、Topo-II、SP2、CyclinD1、CyclinG1、CDK4、CDK5、MMP1、PAR1、RbAp48、Vimentin、N-cadherin的表达量显著高于顺铂敏感的宫颈癌组织;miR-100的表达量与Nek2、P-gp、GST-π、Topo-II、SP2、CyclinD1、CyclinG1、CDK4、CDK5、MMP1、PAR1、RbAp48、Vimentin、N-cadherin的表达量呈负相关。结论:宫颈癌组织中miR-100的低表达与顺铂耐药密切相关。

Objective：To study the relationship between miR-100 expression in cervical cancer tissue and cisplatin resistance.Methods：107cases of cervical cancer tissues from those who received cisplatin chemotherapy in Huanggan Central Hospital between May 2013 and October 2013 were collected as the clinical samples and divided into chemotherapy-responsive cisplatin-sensitive cervical cancer tissue and chemotherapy-irresponsive cisplatin-sensitive cervical cancer tissue according to the curative effect of chemotherapy.The miR-100 expression as well as the expression of drug resistance-related genes,cell cyclerelated genes and cell invasion-related genes was detected.Results：miR-100 expression in cisplatin-resistant cervical cancer tissue was significantly lower than that in cisplatin-sensitive cervical cancer tissue while Nek2,P-gp,GST-π,Topo-II,SP2,CyclinD1,CyclinG1,CDK4,CDK5,MMP1,PAR1,RbAp48,Vimentin and N-cadherin expression were significantly higher than those in cisplatin-sensitive cervical cancer tissue;the miR-100 expression was negatively correlated with Nek2,P-gp,GST-π,Topo-II,SP2,CyclinD1,CyclinG1,CDK4,CDK5,MMP1,PAR1,RbAp48,Vimentin and N-cadherin expression.Conclusion：The lower expression of miR-100 in cervical cancer tissue is closely associated with cisplatin resistance.