优化高效液相色谱法对天然麝香的鉴别

Optimized HPLC Method to Identify the Natural Musk

将已有高效液相色谱(HPLC)法的样品处理过程进行优化改良;采用正交分解法设计实验的色谱条件,确定甲醇与水体积比为90∶10的比例为最优化分离方案;使用改良优化过的HPLC方法分析天然麝香和掺伪麝香的指纹图谱,然后以标准品的数据为参考确认其中是否掺有人工合成麝香酮。结果表明:使用优化后的方法可对天然麝香中是否掺有人工合成麝香酮进行鉴定,并可在一定程度上提高鉴定的准确性、速度与精度。

The experiment was conducted to optimize the sample processing of existing HPLC method, orthogonal decomposition method was used to design chromatographic condition, to determine the optimal separation scheme of methanol/water （90 ： 10 in volumn）. The optimized HPLC was used to identify the fingerprints of natural musk and adulteration musk, with standard data as reference to confirm whether laced with synthetic musk ketone. The optimized method can identify natural musk laced with synthetic musk ketone with good performance in identification accuracy, speed and precision.