摘要
目的:探讨白细胞介素17A(interleukin-17A,IL-17A)rs2275913(G-197A)位点单核苷酸多态性(single nucleotide polymorphisms,SNPs)及血浆水平与重庆汉族人群病毒性心肌炎(viral myocarditis,VMC)的关系。方法:收集VMC患者200例,根据病程将患者分为急性期组112例和恢复期组88例,并选择同期200例健康体检的正常人作为对照组,采用聚和酶链反应-限制性内切酶片断长度多态性(PCR-RFLP)、DNA测序等方法检测全部受试者IL-17A基因rs2275913(G-197A)位点SNP,采用酶联免疫吸附法(ELISA)测定两组血清IL-17水平。结果:VMC组IL-17A基因rs2275913(G-197A)多态位点AA基因型和A等位基因频率均显著高于对照组(P<0.05),携带AA基因型及携带A等位基因(AA+AG基因型)可增加患VMC的易感性(矫正后ORAA=2.197,95%CIAA=1.208-4.279,PAA=0.003;矫正后ORAA+AG=2.051,95%CIAA+AG=1.134-3.995,PAA+AG=0.009)。VMC组血清IL-17水平明显高于对照组(P<0.05),尤以急性期为著(P<0.05)。不论是VMC组还是对照组,IL-17A基因rs2275913(G-197A)多态位点中含A等位基因(AA+AG)者血清IL-17水平均显著高于非A等位基因(GG)携带者(P<0.01)。结论:IL-17A基因rs2275913位点SNP可能与中国重庆地区汉族人群VMC有关,A等位基因是VMC的易感基因。
Objective:To investigate the association of Interleukin-17A(IL-17A)gene rs2275913(G-197A)single nucleotide polymorphism(SNP)and serum IL-17 levels with viral myocarditis(VMC)in Chongqing Han population.Method:The 200 patients with VCM were divided into acute group(112cases)and recovery group(88cases),and another 200 healthy subjects were selected as control group.The rs2275913 polymorphism of IL-17 A was genotyped by polymerase chain reaction-restriction fragment length polymorphism methods and DNA sequence technology.The levels of serum IL-17 were detected by ELISA.Result:The frequencies of IL-17Ars2275913 AA genotypes and A alleles in VMC group were significantly higher than those in control group(P〈0.05).Individuals with AA genotype and the A allele carriers(AA+AG)had significantly increased VMC risk compared with the GG homozygotes(adjusted odds ratio[OR]AA=2.197,95% confidence interval[CI]AA=1.208-4.279,PAA=0.003;adjusted ORAA+AG=2.051,95%CIAA+AG=1.134-3.995,PAA+AG=0.009).The serum level of IL-17 in VMC group was higher than that in the control group,especially for the patients in acute stage(all P〈0.05).The people with A allele(AA+AG)had higher level of serum IL-17 than those only with GG homozygotes in both VMC and control group(all P〈0.01).Conclusion:Our study suggests that the SNP(rs2275913)of IL-17 A gene may be associated with VMC in the Han population of Chongqing,and A allele is a susceptible gene of VMC.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2017年第6期560-563,共4页
Journal of Clinical Cardiology
基金
重庆市科委基础与前沿研究计划项目(No:cstc2015jcyjA10047)