IL-17A基因多态性及血浆水平与病毒性心肌炎的相关性研究

Association of Interleukin-17A gene polymorphism and serum Interleukin-17 levels with viral myocarditis

目的:探讨白细胞介素17A(interleukin-17A,IL-17A)rs2275913(G-197A)位点单核苷酸多态性(single nucleotide polymorphisms,SNPs)及血浆水平与重庆汉族人群病毒性心肌炎(viral myocarditis,VMC)的关系。方法:收集VMC患者200例,根据病程将患者分为急性期组112例和恢复期组88例,并选择同期200例健康体检的正常人作为对照组,采用聚和酶链反应-限制性内切酶片断长度多态性(PCR-RFLP)、DNA测序等方法检测全部受试者IL-17A基因rs2275913(G-197A)位点SNP,采用酶联免疫吸附法(ELISA)测定两组血清IL-17水平。结果:VMC组IL-17A基因rs2275913(G-197A)多态位点AA基因型和A等位基因频率均显著高于对照组(P<0.05),携带AA基因型及携带A等位基因(AA+AG基因型)可增加患VMC的易感性(矫正后ORAA=2.197,95%CIAA=1.208-4.279,PAA=0.003;矫正后ORAA+AG=2.051,95%CIAA+AG=1.134-3.995,PAA+AG=0.009)。VMC组血清IL-17水平明显高于对照组(P<0.05),尤以急性期为著(P<0.05)。不论是VMC组还是对照组,IL-17A基因rs2275913(G-197A)多态位点中含A等位基因(AA+AG)者血清IL-17水平均显著高于非A等位基因(GG)携带者(P<0.01)。结论:IL-17A基因rs2275913位点SNP可能与中国重庆地区汉族人群VMC有关,A等位基因是VMC的易感基因。

Objective：To investigate the association of Interleukin-17A（IL-17A）gene rs2275913（G-197A）single nucleotide polymorphism（SNP）and serum IL-17 levels with viral myocarditis（VMC）in Chongqing Han population.Method：The 200 patients with VCM were divided into acute group（112cases）and recovery group（88cases）,and another 200 healthy subjects were selected as control group.The rs2275913 polymorphism of IL-17 A was genotyped by polymerase chain reaction-restriction fragment length polymorphism methods and DNA sequence technology.The levels of serum IL-17 were detected by ELISA.Result：The frequencies of IL-17Ars2275913 AA genotypes and A alleles in VMC group were significantly higher than those in control group（P〈0.05）.Individuals with AA genotype and the A allele carriers（AA＋AG）had significantly increased VMC risk compared with the GG homozygotes（adjusted odds ratio[OR]AA=2.197,95% confidence interval[CI]AA=1.208-4.279,PAA=0.003;adjusted ORAA＋AG=2.051,95%CIAA＋AG=1.134-3.995,PAA＋AG=0.009）.The serum level of IL-17 in VMC group was higher than that in the control group,especially for the patients in acute stage（all P〈0.05）.The people with A allele（AA＋AG）had higher level of serum IL-17 than those only with GG homozygotes in both VMC and control group（all P〈0.01）.Conclusion：Our study suggests that the SNP（rs2275913）of IL-17 A gene may be associated with VMC in the Han population of Chongqing,and A allele is a susceptible gene of VMC.