6-姜酚抑制氧化应激减轻大鼠心肌缺血/再灌注损伤

6-Gingerol inhibits oxidative stress ameliorated myocardial ischemia/reperfusion injury in rats

目的:研究6-姜酚抑制氧化应激减轻心肌缺血/再灌注损伤(MIRI)的作用与机制。方法:雄性Wistar大鼠40只,按随机原则分为随机分为4组(每组10只):假手术组、6-姜酚组(6-G组)、MIRI组、6-G+MIRI组。6-G组大鼠不行手术,在6-G+MIRI组手术前30min同时经尾静脉给药(6mg/kg)。各组大鼠经ELISA法检测血清丙二醛(MDA)、超氧化物岐化酶(SOD)、心肌酶(CK、CK-MB、TnT、TnI)水平,TUNEL法检测心肌细胞凋亡并计算凋亡指数,TTC双染色法测量心肌梗死面积,ELISA法检测血清Bcl-2、Bax、Caspase-3等水平。结果:与MIRI组比较,6-G+MIRI组MDA水平降低(P<0.05)、SOD水平升高(P<0.05);心肌酶水平下降(P<0.05);心肌细胞凋亡减轻,凋亡指数降低(P<0.05);心肌梗死面积缩小(P<0.05);血清Bcl-2水平升高(P<0.05)、Bax水平下降(P<0.05)、Bcl-2/Bax比值升高(P<0.05)、Caspase-3水平下降(P<0.05)。结论:6-G可减轻MIRI,其机制可能是通过抑制氧化应激减轻心肌细胞凋亡。

Objective：To investigate the protection and mechanism of 6-Gingerol ameliorated myocardial ischemia/reperfusion injury（MIRI）through inhibiting oxidative stress in rats.Method：Forty male Wistar rats were randomly devided into：（1）The Sham-operation group（Sham）;（2）The 6-Gingerol group（6-G）,the rats in this group were not operated,established at 30 min before ischemia with 6-G＋MIRI group（2mg/kg）;（3）The MIRI group,established by blocking left coronary artery anterior descending branch for 30 min followed by repe-rfusion;（4）The 6-G＋MIRI group（6-G＋MIRI）,stablished by tail veins（6mg/kg）at 30 min before ischemia.Then we analyzed the levels of malondialdehyde（MDA）,super-oxide disproportionateon（SOD）enzyme,myocardial enzymes（CK,CK-MB,TnT,TnI）by ELISA.The apoptosis of cardiomyocytes was observed by TUNEL.The areas of myocardial infarction were analyzed by TTC staining.The levels of Bcl-2,Bax,Caspase-3were detected by ELISA.Result：Compared with MIRI group,6-G＋MIRI group significantly reduced serum MDA level（P〈0.05）,increased serum SOD level（P〈0.05）.The apoptosis index were significantly decreased（P〈0.05）.The areas of myocardial infarction were significantly decreased（P〈0.05）.The level of Bcl-2was significantly increased（P〈0.05）,the level of Bax was significantly decreased（P〈0.05）,the ratio of Bcl-2/Bax was significantly increased（P〈0.05）and the level of Caspase-3was significantly decreased（P〈0.05）.Conclusion：6-G ameliorated MIRI,which may be partially related to reduce oxidative stress and myocardial apoptosis.