HPLC法同时测定产康乐合剂中3种成分的含量

Study on Simultaneous Determination of Three Components in Chankangle Mixture(产康乐合剂) by HPLC

目的:建立产康乐合剂中三种有效成分含量测定方法。方法:Agilent TC-C18色谱柱(4.6 mm×250mm,5μm),以0.2%磷酸水溶液和乙腈为流动相(梯度洗脱),进样量10μL,柱温25℃,检测波长为325 nm。结果:绿原酸、黄芩苷、黄芩素分别在7.12~71.18μg/m L;29.39~293.89μg/m L和2.12~21.17μg/m L范围内线性关系良好,平均加样回收率分别为102.61%、103.21%和97.76%(RSD%值分别为1.77%、0.77%和1.48%)。重复性、稳定性、精密度等RSD%均小于2%。结论:该方法简单易行,可用于产康乐合剂质量控制。

Objective： To establish an HPLC method for the determination of three components in Chankangle mixture （产康乐合剂）, Methods： An Agilent TC-C18 column （4.6 mm×250mm, 5 μm） was used. The mobile phase was acetonitrile and 0.2% phosphoric acid solution with gradient elution. The injection volume was 10 μL, the column temperature was 25℃, and the detection wavelength was 325 nm. Results： Chlorogenic acid, baicalin and baicalein had a good linearity at 7.12-71.18μg/mL, 29.39-293.89μg/mL and 2.12-21.17 μg/mL, respectively. The average recoveries and RSD were 102.61% （1.77%）, 103.21% （0.77%） and 97.76% （1.48%）, respectively. The repeatability, stability, and precision RSD was all less than 2%. Conclusion： The methods are simple, and can be used for the quality control of Chankangle Mixture.