摘要
[目的]对日本沼虾胰岛素样雄性腺激素基因(Mn-IAG)进行克隆及生物信息学分析。[方法]用RACE-PCR法获取Mn-IAG基因序列,用生物信息学软件分析该基因及蛋白的结构特征及理化性质。[结果]Mn-IAG基因编码175个氨基酸,该蛋白的分子式为C_(871)H_(1373)N_(255)O_(271)S_(13),理论等电点为6.94;没有跨膜结构域,为亲水性蛋白;存在20个磷酸化位点和2个劈切位点。[结论]Mn-IAG可能与细胞膜上的受体结合,参与日本沼虾的性别调控过程。
[ Objective] To clone mRNA sequences and analyze the bioinformaties of the insulin- like androgenic gland hor- mone （Mn - lAG） from Macrobrachium nipponensis. [ Methods ] The mRNA sequences of Mn - lAG were cloned by RACE - PCR ,the structure characteristics and physicoehemieal property of Mn -IAG mRNA and polypeptide wered analysed by bioin- formatics tools. [ Results] The protein encoded by Mn - IAG was 175 aa in length with the molecular formula was Cs71H1373N255 0271S13 and pI was 6.94 ; IAG was hydrophilie protein without transmembrane domain. There were 20 phosphorylation sites and 2 cleavage sites in IAG peptide chain. [ Conclusion] The Mn - IAG may be invovled in sex regulation of M. nipponere by hind- ing with its receptor of cell membrane reeptors.
出处
《生物技术》
北大核心
2017年第3期259-263,共5页
Biotechnology
基金
山东省自然科学基金面上项目("类胰岛素多肽基因调控日本沼虾生长发育的作用机制"
No.ZR 2016CM12)
山东省高等学校科技计划项目("中华绒螯蟹促雄腺激素基因(Es-IAG)的克隆表达及SNPs分析"
No.J16LE59)
潍坊科技学院博士基金项目("饥饿胁迫下日本沼虾的转录组构建分析"
No.2017BS03)
关键词
日本沼虾
IAG基因
多肽序列
生物信息学分析
Macrobrachium nipponensi, [AG gene, peptide chain, bioinformaties analysis
