周期性流体静压力对BMSCs细胞增殖活性及超微结构的影响

Effects of cyclic hydrostatic pressure on proliferation and ultrastructure of rat's BMSCs

目的:观察周期性流体静压力作用下骨髓间充质干细胞(Bone Marrow Stem cells,BMSCs)细胞增殖活性及超微结构的改变。方法:贴壁法分离培养大鼠BMSCs。采用自主研发的流体静压力加载装置对大鼠BMSCs进行力学加载,加载压力为0~45kPa、0~90kPa,加载频率为0.1 Hz,时间1h,连续加载2d。对照组细胞不加压。采用CCK8法检测细胞的增殖活性,采用透射电镜观察大鼠BMSCs超微结构的变化。结果:0~45kPa、0~90kPa周期性流体静压力作用下BMSCs增殖活性显著高于对照组(P<0.05),透射电镜下观察发现,0~45kPa作用下BMSCs细胞内质网轻度扩张,胶原合成量增加。而0~90kPa作用下细胞出现了大量胶原及凋亡小体。结论:适宜的周期性流体静压力作用下可提高细胞的增殖活性,但过高的压力刺激可诱导细胞产生凋亡。

Objective：Evaluate the proliferation and ultrastructure change of rat’s BMSCs under cyclic hydrostatic pressure.Methods：BMSCs was obtained from rat by the attachment culture method.Mechanical loading of BMSCs was carried out by self-developed hydrostatic pressure loading device.The pressing pressure are 0-45 kPa and 0~90kPa.BMSCs were treated with pressure 1h/d for consecutive 2days,while the controls BMSCs were treated without pressure.Proliferation capabilities of BMSCs were detected by CCK8,while the ultrastructures of BMSCs were examined by transmission electron microscopy.Results：The proliferating activation of BMSCs under cyclic hydrostatic pressure（0-45 kPa and 0-90kPa）was higher in evidence than that of control group（P〈0.05）.The BMSCs treated with 0-45 kPa showed mild expansion of endoplasmic reticulum,and secreted collagen around the cell.However,the BMSCs treated with 0~90kPa showed the character of apoptosis coinciding with rich matrix.Conclusion：Appropriate measure of hydrostatic pressure promotes proliferation of BMSCs,whereas overdue hydrostatic pressure may induce apoptosis of BMSCs.