地涌金莲花加工过程中防褐变技术研究

Investigation of Method against Browning of Musella lasiocarpa Flower during Processing

【目的】本研究旨在探索抑制地涌金莲花在加工过程中褐变的方法。【方法】首次采用柠檬酸、D-异抗坏血酸钠、CaCl_2、EDTA-2Na 4种护色剂对地涌金莲进行护色处理,研究护色剂质量浓度和护色时间对地涌金莲花的色差值、褐变度、多酚氧化酶(PPO)和过氧化物酶(POD)活力的变化。【结果】4种护色剂的护色效果的变化趋势为柠檬酸>D-异抗坏血酸钠>CaCl_2>EDTA-2Na,柠檬酸和CaCl_2的最适护色质量浓度为0.3%,D-异抗坏血酸钠为0.35%;4种护色剂的最适护色时间为2 h;单一柠檬酸的护色效果优于柠檬酸-D-异抗坏血酸钠复合护色剂的护色效果;0.3%柠檬酸处理2 h对PPO活力的抑制率高达96.67%,对POD活力的抑制率高达91.71%。【结论】0.3%柠檬酸处理2 h可以作为一种有效抑制地涌金莲花加工过程中褐变的方法。

[ Purpose ] This research was performed to explore a method against the browning of Musella lasiocarpa flower during process. [ Methods] M. lasiocarpa flower was treated with four color protectors （citric acid, D-sodium erythorbate, calcium chloride and EDTA-2Na） for the firstly time. Chromatic aberration, browning degree, and activity of polyphenol oxidase （PPO） and peroxidase （POD） of M. lasiocarpa flower were measured to investigate the effect of color protectors concentration and treating time. [ Results] The color protection effect of four reagents was ： citric acid 〉 D-sodium erythorbate 〉 calcium chloride 〉 EDTA-2Na. The optimal concentration of citric acid and CaC12 were both at 0.3%, and D-sodium erythorbate was at 0. tectors was 2 h. Citric acid exhibited better color 35%. The optimum soaking time for four color pro- fix effect than its mixture with D-sodium erythor-bate. Processing with 0.3 % inhibition on POD activity. citric acid for 2 h gave 96.67% inhibition on [ Conclusion ] Pretreatment with 0.3 % citric PPO activity, and 91.71% acid for 2 h could be an ef- fective method to prevent the brown of M. lasiocarpa flower during processing.