云青青稞色素提取工艺及体外抗氧化活性测定

Optimization of Extraction Technology and Antioxidant Activities in vitro of Pigments from Yunqing Hulless Barley

【目的】研究云青青稞色素的最佳提取工艺及5种体外抗氧化活性。【方法】以云青青稞为原料,通过二次通用旋转试验设计,优化了云青青稞色素的提取工艺,同时对色素的体外抗氧化活性进行了测定。【结果】云青青稞色素的最佳提取溶剂为1%盐酸甲醇(V_(盐酸)∶V_(甲醇)=1.87∶98.13),最大吸收波长为528 nm,在酸性条件下比较稳定,初步定性为花色苷;该色素最佳提取条件为:提取温度45℃、液料比15∶1(mL∶g)、提取时间40 min;该色素有较好的抗氧化活性,DPPH自由基清除能力为(10.86±2.13)μmol/g,铁离子还原/抗氧化能力为(0.077±0.000 96)mmol/g,总抗氧化能力为(101.48±8.97)μmol/g,还原能力为42.48±14.34μmol/g,H_2O_2清除活性为27.88±13.79μmol/g。【结论】得到云青青稞色素最佳提取工艺,为该色素的开发利用提供科学依据。

[ Purpose] Our aim was to study the optimization of extraction technology and antioxidant activities in vitro of pigments from yunqing hulless barley. [ Method ] The optimal extraction technol- ogy of pigments from Yunqing hulless was investigated by quadratic general rotary design on the basis of single factor experiments. At the same time, the antioxidant activities of pigments were determined u- sing 5 antioxidant activity assay systems in vitro. [ Results ] The results showed that the best extrac- tion solvent for the pigments was 1% hydrochloric acid methanol （ VHc： Vmoho = 1.87 ： 98.13）. Also, the pigments were stability under acid condition and the maximum absorption wavelength was 528 nm. So the pigments may be anthocyanins. The best extraction conditions were as follows： extraction tem- perature was 45 ℃, the ratio of liquid to solid was 15：1 （mL： g）, extraction time was 40 rain. The pig-ments possessed good antioxidant activity. The DPPH free radical scavenging capacity was （ 10. 86 ± 2. 13） μmol/g, ferric reducing/antioxidant capacity was （0. 077 ±0. 000 96） retool/g, total antioxi- dant capacity was （101.48 ±8.97） μmol/g, reduction power was （42.48± 14. 34） μmol/g, 1-120= scavenging activity was （27.88 ± 13.79） μmol/g. [ Conclusion] The optimal extraction process of pigment of yunqing hulless barley is determined. The study provided the scientific basis for the devel- opment and utilization of the pigments.