参薯‘明淮1号’组培快繁技术研究

Study on Rapid Propagation Technology of Tissue Culture of Dioscorea alata 'Minghuai No.1'

【目的】建立参薯‘明淮1号’组培快繁体系。【方法】以参薯‘明淮1号’带腋芽嫩茎为外植体,应用均匀设计和回归分析方法对‘明淮1号’组培的初代培养、继代增殖培养和生根培养等阶段中所需的不同激素进行配比优化研究。【结果】最适初代腋芽诱导培养基为:MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+PVP 100 mg/L+琼脂粉4.0 g/L+蔗糖30 g/L,出芽时间短,诱导率达92%同时可诱导丛生芽形成;最适继代增殖培养基为:MS+6-BA2.33 mg/L+NAA 0.1 mg/L+PP3330.2 mg/L+琼脂粉4.0 g/L+蔗糖30 g/L,增殖系数可达3倍左右;最适诱导生根培养基为:1/2 MS+IBA 0.34 mg/L+IAA 0.5 mg/L+AC 1.0 g/L+琼脂粉4.5 g/L+蔗糖20 g/L,生根率达95%,生根效果好。【结论】该试验结果为‘明淮1号’的推广和产业化发展奠定基础。

[ Purpose ] The aim was to establish rapid propagation of Dioscorea alata ＇ Minghuai No. 1＇ [ Method ] With the tender stems of Dioscorea alata ＇ Minghuai No. 1 ＇ as explants, we carried out different hormone proportioning optimization research of ＇ Minghuai No. 1 ＇ primary culture, prolifera- tion and rooting culture, by uniform design and regression analysis method. [ Results] The optimal axillary buds induction medium was MS ＋ 6-BA 2. 0 mg/L ＋ NAA 0. 1 mg/L ＋ PVP 100 mg/L ＋ agar 4.0 g/L ＋ sugar 30 g/L, the induction time was short and the induction rate was 92% , which could in- duce the formation of cluster buds ; the optimal subculture medium was MS ＋ 6-BA 2.33 mg/L ＋ NAA 0. 1 mg/L ＋ PP333 0.2 mg/L ＋ agar 4.0 g/L ＋ sugar 30 g/L, and the proliferation coefficient could up to 3 times ; The optimal rooting medium was 1/2 MS ＋ IBA 0. 34 mg/L ＋ IAA 0.5 mg/L ＋ AC 1.0 g/L ＋ agar 4.5 g/L ＋ sugar 20 g/L, the rooting rate was 95%, and the rooting effect was good. [ Conclu- sion] The experimental results laid a good foundation for the promotion and industrial development of ＇ Minghuai No. 1＇