黄芪对阿霉素诱导扩张型心肌病大鼠的干预作用

Interventional effects of Astragalus on adriamycin-induced dilated cardiomyopathy in rats

目的探讨黄芪对阿霉素诱导扩张型心肌病(DCM)大鼠的干预作用。方法采用腹腔注射阿霉素成功建立SD大鼠DCM模型后分别实施培哚普利3mg·kg^(-1)·d^(-1)(A组,13只),美托洛尔50mg/kg(B组,12只),黄芪3.5mg·kg^(-1)·d^(-1)(D组,13只)或等体积蒸馏水(E组,10只)干预,连续灌胃给药8周。另取20只SD大鼠作为空白对照(C)组。检测指标:心脏超声检查,ELISA法测定大鼠血清脑钠肽(BNP)水平,取大鼠左心室心肌行HE染色,RT-PCR法测定心肌组织Bax、Bcl-2、FasL、Fas、Caspase-3 mRNA水平;C、D、E组行心肌代谢显像检查。结果与E组比较,A、B、D组大鼠左心室舒张末期内径、左心室收缩末期内径及血清BNP下降(P<0.01),左心室射血分数和短轴缩短率升高(P<0.01)。HE染色示A、B、D组大鼠心肌纤维化程度有改善。与E组比较,A、B、D组Bax、FasL、Fas、Caspase-3 mRNA表达下降,而Bcl-2 mRNA表达升高(P<0.05或P<0.01),D组大鼠最大标准化摄取值升高(5.97±0.53vs.1.04±0.28)(P<0.01)。结论黄芪能改善心肌活性。其作用机制可能与调节心肌细胞凋亡的线粒体途径和/或死亡受体途径相关因子表达相关。

Objective To explore the interventional effects of Astragalus on adriamycin-induced dilated cardiomyopathy（DCM）in SD rats.Methods Using adriamycin 1.0mg/kg,twice per week for6 weeks,DCM model rats were established and treated with perindopril 3mg·kg-1·d-1（group A,13rats）,metoprolol 50mg/kg（group B,12rats）,Astragalus 3.5 mg·kg-1·d-1（group D,13rats）or equivolumne distilled water（group E,10rats）.Another 20 SD rats were taken as the blank controls（group C）,which were lavaged intragastrically twice a day for 8weeks.The parameters detected were cardiac ultrasonography,serum brain natriuretic peptide（BNP,by ELISA）,HE staining of left ventricular muscles,the expressions of Bax,Bcl-2,FasL,Fas and Caspase-3 mRNA in cardiac muscles（by RT-PCR）.Myocardial metabolic imaging examination was performed in groups of C,D and E.Results Compared with group E,left ventricular end diastolic dimension,left ventricular end systolic dimension and serum BNP were decreased,while left ventricular ejection fraction and fractional shortening were increased in groups of A,B and D（P〈0.01）.HE staining showed that myocardial fibrosis was improved in groups of A,B and D.Compared with group E,the expressions of Bax,FasL,Fas and Caspase-3 mRNA were decreased,but Bcl-2 mRNA expression was increased in groups of A,B and D（P〈0.05 or P〈0.01）.The standardized uptake value was higher in group D than that in group E（5.97±0.53 vs.1.04±0.28）（P〈0.01）.Conclusion Astragalus can effectively improve heart function of DCM rats,which may be related to the relevant factors of mitochondrial pathway or death receptor pathway for the regulation of myocardial cell apoptosis.