血液标本溶血干扰与影响生化检验结果研究

Study of interference and influence of blood specimen hemolysis in biochemical test results

目的 研究血液标本溶血干扰与影响生化检验结果.方法 选取杭州市富阳区第二人民医院2014年5月-2016年5月接收的健康体检人员80例,依据血液标本溶血发生情况将这些人员分为溶血组（n=40）和未溶血组（n=40）,2组人员于清晨空腹采集静脉血5mL,在肝素抗凝管中放置,溶血组人员的血液经竹签搅动,受到诱导发生溶血;未溶血组人员的血液不经上述处理,直接以2500r/min的速率进行10min的离心,将血清分离出来,采用全自动生化反应分析仪对2组人员的K＋、GLU、ALB、TP、CK、TBIL、BUN、ALT、AST、LDH水平进行检测,然后对2组人员的各项生化指标水平进行统计分析.结果 溶血组人员的GLU水平显著低于未溶血组（P〈0.05）,K＋、TP、CK、TBIL、AST、LDH水平均显著高于未溶血组（P〈0.05）,但2组人员的ALB、BUN、ALT水平之间差异均无统计学意义.结论 血液标本溶血会在一定程度上干扰与影响生化检验结果.

Objective To study the interference and influence of blood specimen hemolysis in biochemical test results.Methods80 cases of personnel received physical examination in our hospital from May 2014 to May 2016 were selected, these personnel were divided into hemolytic group （n=40） and non hemolytic group （n=40） on the basis of blood specimen hemolysis occurred situations, 5mL morning fasting venous blood of the two groups was collected, they were placed in heparinized tubes, the blood of the hemolytic group was stirred by bamboo inducing hemolysis;The blood of the non hemolysis group was without the above treatment, directly at the rate 2500r/min 10min centrifugal separating serum, the K＋, GLU, ALB, TP, CK, TBIL, BUN, ALT, AST, LDH levels of the two groups were detected by automatic biochemical reaction analyzer, and then the biochemical indicators levels of the two groups were statistically analyzed.ResultsThe level of GLU in the hemolysis group was significantly lower than that in the non hemolytic group （P〈0.05）, the K＋, TP, CK, TBIL, AST, LDH levels in the hemolysis group were significantly higher than those in the non hemolytic group （P〈0.05）, but the differences of ALB, BUN, ALT levels between the two groups were not significant.ConclusionBlood specimen hemolysis can interfere and influence the biochemical tests results to some extent.