高效液相色谱法测定多组分样品的实验教学探讨

Experimental Teaching of HPLC with UV Switch Simultaneous Determination of Multicomponent Contents

高效液相色谱法是天然有机化合物定性定量分析测试的重要手段。该文探讨建立一种采用RP-HPLC同时测定丹皮中几种单萜苷类化合物含量的方法。研究确定了HPLC法分离和测定的色谱条件,包括色谱柱、流动相、流速、检测波长等。该实验为课题组实际研究过程中多组分样品的分析,实验过程中采用流动相梯度洗脱和测试波长变换测试的方法,实验方案的设计涵盖了高效液相色谱法测试中的主要知识点。实践表明,通过实验提高了学生的学习兴趣,锻炼了动手能力。

High performance liquid chromatography （HPLC） method is one of the important methods for native compounds analysis. This paper establishes an HPLC method for simultaneous determination of six monoterpene glycosides. Chromatographic condition was optimized, which included column, mobile phase, flow rate, detection wavelength. The experiment is a muhieomponent analysis in the actual research process of the research group. The research process includes the gradient elusion and the various wavelength test. The design of the experimental scheme covers the main knowledge points of high performance liquid chromatographic test. The practice shows the students interest in learning and the ability of practicing are all improved through the experiment.