摘要
目的:建立测定三七药酒中补骨脂素与异补骨脂素含量的方法。方法:采用高效液相色谱法。色谱柱为依利特Sino Chrom ODS-BP(4.6 mm×200 mm,5μm),流动相为水-甲醇-乙腈(60:37:3),流速为1.0 mL·min^(-1),柱温为25℃,检测波长为246 nm,进样量为10μL。结果:补骨脂素与异补骨脂素的检测质量浓度分别在2.392~191.329μg·mL^(-1)、2.534~202.720μg·mL^(-1)范围内与各自峰面积积分值呈良好的线性关系(r=0.999 9和0.999 9);精密度、稳定性、重复性试验的RSD≤0.47%;平均回收率分别为99.46%、100.42%,RSD分别为0.15%、0.25%(n=6)。结论:本方法简洁快速、灵敏度高、专属性强、结果稳定,可用于三七药酒中补骨脂素与异补骨脂素的含量测定。
Objective： To establish a method for determination of the contents of psoralen and isopsoralen in Panax Notoginseng Wine. Methods： HPLC method was adopted. The determination was performed on Elite SinoChrom ODS-BP column （4.6mm×200mm,5μm） with a mobile phase of water-methanol-acetonitrile （60： 37：3） at a flow rate of 1.0 mL·min-1. The column temperature was 25℃, and the detection wavelength was set at 246 nm. The injection volume was 10 μL. Results： The linearity was good with the ranges of 2. 392-191. 329 μg· mL-1 for psoralen（r = 0. 999 9）and 2. 534-202. 720 μg· mL-1 for isopsoralen （r = 0. 999 9 ）. The RSDs of precision, stability and repeatability were lower than 0. 47%. The average recoveries for Psoralen and Isopsoralen were 99.46% with RSD as 0. 15% （n =6） and 100. 42% with RSD as O. 25% （n =6） respectively. Conclusion： The simple method has the high sensitivity and good specificity which can be used to determine the content of psoralen and isopsoralen in Panax Notoginseng Wine.
出处
《中国药品标准》
CAS
2017年第3期202-206,共5页
Drug Standards of China
