无溶剂体系酶法制备富含甘油二酯稻米油的研究

Study on solvent-free enzymatic preparation of rice oil enriched diacylglycerol

以高酸值稻米油和单甘酯为原料,在单因素试验的基础上,利用P1ackett-Burman试验对各因素进行筛选,得出反应时间、反应温度、酶添加量是影响酶法制备富含甘油二酯稻米油工艺的主要因素;再利用Box-Behnken响应面法优化,得到最佳工艺条件为反应时间9.7h、反应温度67℃、酶添加量3%,在此工艺条件下,稻米油中甘油二酯的含量达到46.68%。

High acid value of rice oil and monoglycerid were used as raw material. On the basis of single factor, various factors were selected by Plackett-Burman test. The results showed that reaction time, reaction temperature and enzyme dosage were the main influencing factors of enzymatic preparation of rice oil enriched diacylglycerol. The Box-Behnken response surface method was used to optimize the process conditions, The optimum conditions were as follows： reaction time 9.7 h, reaction temperature 67℃ and enzyme dosage 3%. Under these conditions, diacylglycerol reached 46.68% in rice oil.