响应面法优化八角茴香中莽草酸的微波提取工艺

Optimization of microwave assisted extraction process of shikimic acid from Illicium verum Hook.f. by response surface analysis method

采用改进的真空微波提取设备,微波提取同时适当抽真空,研究微波辅助提取法提取八角茴香中的莽草酸,在单因素试验的基础上,采用响应面分析法对提取工艺进行优化。以莽草酸的提取率为指标,研究了微波功率、微波时间、液料比和原料粒度对莽草酸提取率的影响。用Box-Behnken设计方法对微波功率、微波时间和液料比进行3因素3水平的优化试验,确定微波辅助提取莽草酸的最佳工艺条件为微波功率500 W、微波时间15.65 min、液料比30:1(mL/g)时,提取2次,得到莽草酸的提取率为20.70%。

Based on single-factor experiments, the response surface analysis were adopted to optimize the microwave-assisted extraction of shikimic acid from Illicium vemm Hook.f. by using the improved vacuum microwave extraction equipment, which microwave extraction and vacuum extraction were carried out at the same time. Microwave power, microwave time, liquid-to-solid ratio and particle size were studied in the single-factor experiments. Base on the Box-Behnken design, microwave time, microwave power and the liquid-to-solid ratio were chosen as the three affecting factors. And the process condition were optimized by response surface method. The optimum extraction condition was obtained as follows： microwave power 500 W, microwave time 15.65 min and liquid-to-solid ratio 30 ： 1 （mL/g） for two times. Under optimized operating conditions, extraction rate of shikimic acid was 20.70%.