琼胶降解菌FG12的全基因组测序

Whole Genome Sequencing of Agar-degrading Strain FG12

为了获得琼胶寡糖的高产琼胶降解菌,本研究通过Illumina Hiseq2000平台测序,使用SOAPdenovo2.04软件(拼接组装),Glimmer 3.02预测基因的开放性阅读框,RNAmmer 1.2预测rRNA,tRNAscan-SE 1.23预测tRNA以及COG、CO和KEGG等来预测FG12的基因功能,并对其进行了全基因组测序,结果表明:FG12的基因组大小为4.11Mb,GC含量为37.76%;共有4 441个开放性阅读框,其平均长度为780 bp,有76个tRNA和5个rRNA;有与琼胶寡糖代谢相关的基因和抗生素的代谢途径,因此得出,琼胶降解菌FG12有改造成为高效工程菌的潜力.

To obtain agar-degrading strain high-producing agarooligosaccharide, the genome of FGI2 was sequenced on the Illumina Hiseq2000 sequencing platform. SOAP denovo 2.04 was used for assembling. Glimmer 3.02 was used to predict open reading frame of the gene. RNAmmer 1.2 was used to predict rRNA. tRNAscan- SE 1.23 was used to predict tRNA. Gene function was annotated by COG, CO, and KEGG databases. The resuits showed that the genome size of FG12 was 4.11 Mb with the G ＋ C content of 37.76%, containing 4,441 open reading frames （ORFs）, the average length of which was 780 bp, and which included 76 tRNA and 5 rRNA. Agar oligosaccharide metabolism-related genes and metabolic pathways of antibiotics were observed. These data suggested that Agar-degrading bacteria FG12 has the potential to be transformed into efficient engineering bac.teria.