青海藏族和回族胃癌发病风险与CYP2E1基因多态性

Risk of Gastric Cancer and Polymorphism of CYP2E1 Gene in Tibetan and Hui at Qinghai

目的明确青海地区藏、回族胃癌患者CYP2E1基因SNP位点分布频率,探讨青海地区藏、回族胃癌患者CYP2E1基因SNP与环境因素的相关性。方法在青海地区藏、回族病例中随机选取研究对象,运用病例对照研究,按照随机数字表法分为病例组和对照组,各96例,全血抽提基因组DNA。采用聚合酶链式反应(Polymerase Chain Reaction,PCR)、变性高效液相色谱分析(denaturing high performance liquid chromatography,DHPLC)方法研究青海藏族、回族CYP2E1基因多态性(Single Nucleotide Polymorphisms,SNP)及与胃癌的相关性。病例组与对照组之间位点基因型频数分布差异采用χ2检验。同期进行病例组与对照组间胃癌高危因素调查。结果 1.CYP2E1基因5'-端位点在藏族病例组和对照组的分布频率分别为c1/c1:80.2%、c1/c2:13.5%、c2/c2:6.3%;c1/c1:82.3%、c1/c2:15.6%、c2/c2:2.0%(P>0.05);回族病例组和对照组的分布频率分别为40.6%、52.1%、7.3%;71.88%、22.92%、5.2%(P<0.05)。结论 1.CYP2E1基因不是青海藏族人群胃癌的易感基因;2.CYP2E1基因是青海回族人群胃癌的易感基因;3.年龄、幽门螺旋杆菌、饮食习惯、高脂血症、慢性萎缩性胃炎、家族胃癌病史是青海地区藏族人群罹患胃癌的主要环境危险因素;4.年龄、大量进食肉类、幽门螺旋杆菌、慢性萎缩性胃炎、血小板数异常是青海地区回族人群罹患胃癌的主要环境风险因素。

Objective To check the frequency of Qinghai Tibetan and Hui CYP2E1 in patients with gastric carcinoma SNP gene frequency distribution, and investigate the correlation between CYP2E1 gene SNP and environ- mental factors in Tibetan and Hui patients with gastric cancer at Qinghai province.Methods In the case of Tibetan and Hui nationality in Qinghai area, the subjects were randomly selected, the case control study was divided into the case group and the control group according to the random number table, each with 96 cases, Whole blood extraction genomic DNA.PCR and DHPLC were used to study the correlation between CYP2E1 SNP and gastric cancer,The difference of genotype frequency distribution between the case group and the control group was analyzed by X2 test. The high-risk factors of gastric cancer between case group and control group were investigated at the same time.Re- suits The frequencies of the 5＇-CYP2E1 gene in Tibetan case group and control group were c 1/el ：80.2%,cl/c2： 13.5%, c2/c2 ： 6.3% ; cl/cl ： 82.3%, cl/c2 ： 15.6%, c2/c2 ： 2.0%, respectively. （ P〉0.05 ） ; The frequency distribu- tion of the case group and the control group was 40.6%, 52.1%, 7.3% ; 71.88%, 22.92%, 5.2%, respectively （P〈0.05） .Conclusions 1.CYP2E1 gene is not a susceptible gene of gastric cancer in Qinghai Tibetan population.2. CYP2E1 gene is a susceptible gene of gastric cancer in Hui people in Qinghai.3.Age, Helicobacter pylori, dietary habits, hyperlipidemia, chronic atrophic gastritis and family history of gastric cancer are the main environmental risk factors for gastric cancer in Tibetan population in Qinghai area.4. Age, large amount of meat, Helicobacter pylori, chronic atrophic gastritis and abnormal platelet count are the main environmental risk factors for gastric cancer in Hui people in Qinghai area.