活动性结核病患者外周血单个核细胞中结核分枝杆菌抗原特异性多能CD4＋和CD8＋T淋巴细胞的特征研究

Identified the Characteristic of M. tb-specific CD4^+ T and CD8^+ T Cells Responses Induced in PBMCs of Active Tuberculosis Patients

目的研究活动性结核病患者外周血单个核细胞中结核分枝杆菌抗原特异性多能T淋巴细胞细胞因子的分泌特征。方法利用γ干扰素释放试验和多色流式细胞术分析了13例活动性结核病患者、11例肺部感染性/肿瘤疾病患者以及14例健康对照者外周血中结核分枝杆菌抗原特异性(ESAT-6和CFP-10)CD4^+Th1和CD8^+Tc淋巴细胞表达细胞因子IFN-γ、TNF-α和IL-2的情况。结果与肺部感染性/肿瘤疾病组和健康对照组相比:(1)活动性结核病组具有较低比例的分泌TNF-α^+的CD4^+Th1细胞、较高比例的分泌IFN-γ^+和IFN-γ^+TNF-α^+IL-2^+的CD4^+Th1细胞;(2)活动性结核病组具有较高比例的分泌IFN-γ^+TNF-α^+IL-2^+的CD8^+Tc细胞。结论实验结果提示活动性结核病患者中表达IFN-γ^+TNF-α^+IL-2^+的多能CD4^+Th1及CD8^+Tc细胞,可能在区别活动性结核病与肺部感染性/肿瘤疾病方面具有一定的临床参考价值。

Objective To evaluated the characteristic of cytokines of multifunctional CD4＋ and CD8＋ T cells induced by M.tb-specific antigen.Methods Total of 13 active tuberculosis patients with positive ELISpot, 11 pulmonary infection/tumor disease patients and 14 healthy controls with negative ELISpot were enrolled.Qualitative analysis of M.tb-specific CD4＋Th1and CD8＋Tc cells responses （including IFN-γ, TNF-α and IL-2） were done with polychromatic flow cytometry after stimulation with the specificity of Mycobacterium tuberculosis antigen （ESAT-6 and CFP-10）.Results Compared with the pulmonary infection/tumor disease group and the healthy control group： （1） the active tuberculosis group had a lower proportion of CD4＋ Th1 cells secreted TNF-α, a higher proportion of CD4＋ Th1cells secreted IFN-γ or IFN-γ, both TNF-α and IL-2;（2） CD8＋Tc cells secreted IFN-γ, TNF-α and IL-2 coincidently was higher in the active tuberculosis group.Conclusion Our results indicated that multifunctional CD4＋Th1 and CD8＋Tc cells expressing IFN-γ, TNF-α and IL-2 concurrently may be associated with clinical reference value in active tuberculosis patients.