摘要
运用荧光猝灭光谱、分子对接等手段研究了表没食子儿茶素没食子酸酯(EGCG)与牛乳β-乳球蛋白之间的相互作用及作用机理。首先采用荧光光谱法研究了EGCG与β-LG的结合反应,并测定了结合常数,然后再利用Autodock 4.0软件对EGCG与β-LG的分子对接进行了研究。结果表明EGCG与β-LG形成复合物从而猝灭β-LG的内源荧光,其荧光猝灭机理符合静态机制,在17℃、37℃下的结合常数分别为2.313×104L/mol、1.507×10~4L/mol。热力学数据表明,EGCG与β-LG存在疏水作用、氢键及静电作用,分子对接结果与荧光猝灭结果基本吻合。
In the present work, the interaction of EGCG and β-1actoglobulin was studied by fluorescence quenching spectra and molecular docking. The results showed that these interactions result in the endogenous fluorescence quenching of β-lactoglobulin,which belongs to a static quenching mech- anism. The calculated bind constants were 2. 313 × 10^4 L/mol and 1. 507× 10^4 L/mol at 17 ℃ and 37 ℃, respectively. The results revealed that quercetin tended to bind with β-1actoglobulin mainly by hydrogen bond and hydrophobic interaction, as well as electrostatic force. The result of molecular docking was identical essentially with the flurescence quenching＇s.
作者
金建昌
王石磊
刘彩琴
王楠
杜琪珍
JIN Jianchang WANG Shilei LIU Caiqin WANG Nan DU Qizhen(School of Biology and Environmental Engineering, Zhejiang Shuren Univertiy, 310015, Hangzhou, PRC School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University ,311300, Lin＇an, Zhejiang, PRC)
出处
《江西科学》
2017年第3期343-346,共4页
Jiangxi Science
基金
浙江省教育厅项目(Y201225599)
浙江树人大学"校中青年学术团队项目"
