大鼠脑β-淀粉样蛋白表达及神经行为学与高胆固醇水平的关系

The relationship between the expression of brain amyloid beta protein along with neural behavior in rats with hypercholesterolemia level

目的研究大鼠脑β-淀粉样蛋白表达及神经行为学与高胆固醇水平的关系,以明确辛伐他汀对阿尔茨海默病(AD)防治有一定作用。方法将32只健康Wistar大鼠按随机数字表法分为普通组和高脂组,每组16只。饲养2周后,再将普通组随机分为普通药物组和普通安慰剂组,每组8只;将高脂组大鼠随机分为高脂药物组、高脂安慰剂组,每组8只。药物组16只大鼠给予辛伐他汀灌胃,安慰剂组给同体积安慰剂(蒸馏水)灌胃。免疫组织化学法测定脑内β-淀粉样蛋白(Aβ)蛋白的表达,Morris水迷宫实验测试大鼠的学习和记忆能力。结果高脂药物组大鼠前额皮层Aβ_(1-42)的蛋白表达较高脂安慰剂组减少(P<0.05);高脂药物组与普通安慰剂组、普通药物组之间比较,差异无统计学意义(P>0.05)。与高脂安慰剂组比较,高脂药物组大鼠逃避潜伏期缩短(P<0.05),穿台次数增多(P<0.05);高脂药物组与普通安慰剂组、普通药物组之间比较,差异无统计学意义(P>0.05)。结论辛伐他汀对高胆固醇血症大鼠前额皮层Aβ_(1-42)的蛋白表达有一定影响,同时伴随大鼠神经行为学改变。

Objective To research the relationship between the expression of brain amyloid beta （AI3） protein along with neural behavior in rats with hypercholesterolemia level, in order to clarify the preventive effect of simvastatin on Alzheimer＇s disease. Methods The 32 Wistar rats were divided into normal group and high cholesterol group according to random number table, with 16 rats in each group. After two weeks, the rats in the normal group were randomly divid- ed into normal drug group and normal placebo group, with 8 rats in each group; and the rats in the high cholesterol group were randomly divided into high fat drug group and high fat placebo group, with 8 rats in each group. And then simvastatin were filled into the stomach of the 16 drug group rats, the same volume placebo （distilled water） were filled into the stomach of placebo group. Immunohistochemical method was used to determine the expression of Aβ protein in brain, Morris water maze was used to test the cognitive and memory abilities in rats. Results Aβ1-42 protein expression of the prefrontal cortex in the high fat drug group was less than that of high fat placebo group （P 〈 0.05）; and there was no significant difference in the high fat drug group, normal placebo group and normal drug group （P 〉 0.05）. In the high fat drug group, the escape latency was decreased than that of high fat placebo group （P 〈 0.05）, times of crossing platform was increased （P 〈 0.05）; and there was no significant difference in the high fat drug group, normal placebo group and normal drug group （P 〉 0.05）. Conclusion Simvastatin has some effects on the expression of Aβ1-42 protein in prefrontal cortex of hypercholesterolemic rats, and accompanied by neurobehavioral changes in rats.