miR-21表达量与动脉粥样硬化程度的关系及长春西汀对其影响

Relationship between the expression quantity of miR-21 and the degree of atherosclerosis and the influence of Vinpocetine on miR-21

目的研究不同程度动脉粥样硬化与miR-21表达量的相关性,以及应用长春西汀后对miR-21表达量的影响。方法选取2014年1月~2016年12月于首都医科大学附属北京友谊医院医疗保健中心住院的动脉粥样硬化患者40例,按动脉粥样硬化程度将其分为轻度(9例)、中度(6例)和重度(25例),按治疗方法将其分为用药组(17例)和对照组(23例)。提取患者的血清RNA并利用实时定量PCR方法分析不同程度动脉粥样硬化患者血清中miR-21水平,比较用药组和对照组治疗前后miR-21含量和血细胞比容的变化。结果重度动脉粥样硬化患者血清miR-21表达水平明显高于轻度动脉粥样硬化患者,差异有统计学意义(P<0.05)。治疗后,用药组血细胞比容、miR-21相对含量均较治疗前明显下降,差异有统计学意义(P<0.05),用药组和对照组治疗后miR-21相对含量比较,差异无统计学意义(P>0.05)。结论动脉粥样硬化患者血清miR-21水平随动脉粥样硬化程度的增高而增高,经长春西汀治疗后,miR-21表达水平明显下调,提示miR-21与动脉粥样硬化程度相关。

Objective To study the correlation between different degrees of atherosclerosis and the expression quantity of miR-21, and the influence of Vinpocetine application on the expression quantity of miR-21. Methods Forty patients with atherosclerosis hospitalized in Medical Care Center, Beijing Friendship Hospital, Capital Medical University from January 2014 to December 2016 were selected, and they were divided into mild（9 cases）, moderate（6 cases） and severe（25 cases） according to the degree of atherosclerosis, and they were also divided into medication group（17 cases） and control group（23 cases） according to the treatment method. The serumRNA of patients was extracted and the levels of serum miR-21 in the patients with different degrees of atherosclerosis were analyzed by real-time quantitative PCR,the changes of miR-21 and haematocrit before and after treatment in the medication group and control group were compared. Results The expression of miR-21 in patients with severe atherosclerosis was higher than that in patients with mild atherosclerosis, the difference was statistically significant（P〈0.05）. After treatment, the haematocrit and relative contents of miR-21 in the medication group were lower than those before treatment, the differences were statistically significant（P〈0.05）, there was no statistically significant difference of the relative contents of miR-21 after treatment between medication group and control group（P〉0.05）. Conclusion The levels of serum miR-21 in patients with atherosclerosis are increased with the increase of atherosclerosis, and the expression of miR-21 is significantly decreased after treatment with Vinpocetine, which indicates that miR-21 has correlation with atherosclerosis.