摘要
为了明确河南郑州某高尔夫球场的多年生黑麦草(Lolium perenne)上发生的病害种类,通过田间调查对草坪病害症状进行描述,对病样进行保湿培养并对分离到的病原物进行形态学观察、核糖体DNA-ITS序列分析。结果表明:该病原真菌的形态学与已报道的稻梨孢菌(Pyricularia grisea)相同,核糖体DNA-ITS序列测定分析该菌与稻梨孢菌的同源性达到100%,可以确定该球场的多年生黑麦草上的病害为稻梨孢菌引起的草坪灰斑病。
This study was to define the symptom and causal agent of the Loliurn perenne samples taken from a golf course in Zhengzhou, Henan Province. We observed the symptom of the disease and isolated the pathogen. The morphological characteristics and the DNA sequence of ribosomal ITS of the pathogen were studied. The pathogen was morphologically identified as Pyricularia grisea. The DNA sequence of ribosomal ITS analysis showed that it was 100% similar to that of P. grisea downloaded from Genbank. Thus we could confirm that the disease on Loliurn perenne samples was gray leaf spot caused by P. Grisea.
出处
《草地学报》
CAS
CSCD
北大核心
2017年第2期442-444,共3页
Acta Agrestia Sinica
基金
973国家重点基础研究计划课题(2014CB138706)
教育部创新团队"草地农业系统耦合与管理"(IRT13019)
公益性行业(农业)科研专项(201403071-6)资助
关键词
草坪草
多年生黑麦草
稻梨孢菌
灰斑病
Turfgrass
Lolium perenne
Pyricularia grisea
Gray leaf spot
