miR-451a对人滋养细胞迁移、侵袭能力的影响

The effect of miR-451a on the migration and invasion of human normal trophoblast cell

目的研究miR-451a对人滋养细胞系(HTR-8/SVneo)细胞迁移、侵袭能力的影响。方法以人工合成的寡核苷酸miR-451a模拟物(miR-451a mimics)、miR-451a抑制物(miR-451a inhibitor)分别转染人滋养细胞,过表达与特异性抑制miR-451a;应用逆转录-实时荧光定量聚合酶链反应技术(q RT-PCR)检测转染后miR-451a的表达;应用划痕实验和Transwell小室侵袭实验观察转染前后细胞的迁移和侵袭能力的变化。结果对比空白组及阴性对照组,人滋养细胞转染miR-451a mimics后,miR-451a表达明显升高,细胞迁移、侵袭能力下降;而转染miR-451a inhibitor后,miR-451a表达明显下降,细胞迁移、侵袭能力提高。结论 miR-451a可以抑制人滋养细胞的迁移、侵袭能力。

Objective To investigate the effect of miR-451a on the migration and invasion ability of human normal trophoblast cell line（HTR-8/SVneo）. Methods The synthesized miR-451a mimics and miR-451a inhibitor was transfected into HTR-8/SVneo cells, to get expression and specific inhibition of miR - 451 a. The expression of miR-45 la was identified by real-time PCR after transfection. The cell migration ability was detected by Wound-healing assay, and the cell invasion ability was detected by Transwell assay. Results Compared to blank control group and negative control groups, the expression of miR-451a was upregulated significantly in miR-451a mimics group,with the cell migration ability and invasion ability decreased; the expression of miR-451 a was down-regulated significantly in miR-451 a inhibitor group, with the cell migration ability and invasion ability increased. Conclusion The migration and invasion ability of human normal trophoblast cell line could be inhibited by miR-451 a.