不同时程MPTP造模小鼠焦虑症状的评价

The evaluation of anxious symptoms in different process models of mice induced by MPTP

目的 比较同剂量的1-甲基-4-苯基-1,2,3,6-四氢吡啶制作不同帕金森病模型小鼠的焦虑症状.方法 将60只8周龄C57BL/6雄性小鼠随机分成6组：急性模型组、急性对照组、亚急性模型组、亚急性对照组、慢性模型组、慢性对照组,每组10只.模型组每次均以20 mg·kg^-1的剂量注射1-甲基-4-苯基-1,2,3,6-四氢吡啶,急性模型组连续腹腔注射2 d（4次·d^-1）,亚急性模型组连续腹腔注射8 d（1次·d^-1）,慢性模型组连续腹腔注射4周（每周2次）;同时相应对照组以等量生理盐水腹腔注射.造模结束,使用高架十字迷宫和明暗箱检测小鼠的焦虑症状. 结果 高架十字迷宫实验：小鼠进入开臂次数与总入臂次数的百分比及在开臂内运动时间与在开臂闭臂内总时间的百分比,急性模型组与急性对照组比较差异无统计学意义（P〉0.05）,亚急性模型组、慢性模型组显著低于相应对照组（P〈0.05或0.01）.明暗箱实验：模型组小鼠进入暗箱次数显著少于相应对照组（P〈0.05或0.01）,停留在暗箱的时间显著长于相应对照组（P〈0.05或0.01）.结论 使用1-甲基-4-苯基-1,2,3,6-四氢吡啶亚急性、慢性造模出现的焦虑症状一致性好,是帕金森病伴焦虑障碍的理想模型.

Objective To compare anxious symptoms in different process models of Parkinson＇s disease （PD） mice induced 1-methyl-4-phenyl-1,2,3, 6-tetrahydropyridine （MPTP）.Methods Sixty male C57BL / 6 mice aged 8 weeks were randomly divided into six groups： acute model group （AM）, acute control group （AC）, subacute model group （SM）, subacute control group （SC）, chronic model group （CM） and chronic control group （CC） of 10 ones each.All model groups were injected with MPTP 20 mg/kg/time, AM continuously for 2 days （4 times/day）, SM for 8 days （1 time/day）, and CM for 4 weeks （twice/week）;corresponding control groups were peritoneally injected equivalent physiological saline simultaneously.After the MPTP injection anxious symptoms were tested using Elevated plus-maze （EPM） and Light-dark box （LDB）.Results There were no significant differences in OEr% and OTr% between AM and AC group （P〉0.05）, those were significantly lower in SM and CM than in corresponding control groups （P〈0.05 or 0.01）.The number of entries into the dark compartment was significantly fewer （P〈0.05 or 0.01） and the time spent in the dark compartment more （P〈0.05 or 0.01） in model than in corresponding control groups.Conclusion The consistency of anxious symptoms of SM and CM mice induced by MPTP is good, which are ideal models of PD accompanied with anxiety disorders.