新生大鼠心脏再生模型的改良及评价模式

Improvement and preliminary evaluation of neonatal rat cardiac regeneration model

探索改良新生大鼠心脏再生模型并进行初步评价,以优化哺乳动物在体心脏再生模型.取出生12 h左右的大鼠,采用低温麻醉行心尖切除改良术,术后统计1,3,7和21 d存活率,并取材测量切除的心尖横截面积来评估改良术的效果.术后1 d对照组存活率为61%,改良组为77%,并且改良组术后3,7,21 d的存活率都高于对照组.改良组心尖切除的横截面积变异较小,说明新生大鼠心脏再生模型改良术均一稳定,手术成功率高,可用于哺乳动物心脏再生相关基因和信号通路的研究.

An improved neonatal rat cardiac regeneration model was established and a preliminary evaluation was conducted. Neonatal rats of 12 h and exercised apical resection surgery with low-temperature anesthesia were used. Postoperative survival rates were taken on 1, 3, 7 and 21 d, and the cross-sectional area were measured to evaluate the advantage of the improved technique. In the control group, postoperative survival rate at 1 d was 61%, while the improved group was 77%. In addition, survival rates of the improved group on 3, 7 and 21 d were higher than that in the control group. Variation of resected apex crosssectional area was smaller in the improved group. In conclusion, the improved neonatal rat cardiac regeneration model is more stable and more uniform with a higher operation success rate. This model may be used to investigate genes and the signaling pathways mediating mammalian heart regeneration.