OIP5基因在肾癌细胞中的作用及索拉非尼对Raf1/OIP5通路的影响

Role of OIP5 gene in renal cancer and effect of Sorafenib on Raf1/OIP5 pathway

目的探讨OIP5基因在肾癌中的作用机制及索拉非尼对Raf1/OIP5通路的影响。方法利用CCK-8及细胞迁移实验来检测上调OIP5基因前后细胞的增殖活力及迁移能力,并利用侵袭实验来检测下调OIP5基因前后细胞的侵袭能力。在786-O细胞中下调Raf1基因后,检测OIP5基因的RNA及蛋白的表达情况。利用5μmol/L和10μmol/L的索拉非尼作用于786-O细胞48h后,检测Raf1和OIP5基因的表达情况。结果上调OIP5基因后786-O细胞的增殖活力及迁移能力都明显增强。下调OIP5基因后786-O细胞的侵袭能力明显减弱。下调Raf1基因后,OIP5基因的RNA及蛋白水平都明显下调。利用5μmol/L和10μmol/L的索拉非尼作用于786-O细胞48h后,Raf1及OIP5的表达均明显下调。结论 OIP5基因在肾癌中起到类似癌基因的作用,索拉非尼可以通过Raf1/OIP5通路发挥其抗肿瘤的作用。

Objective To explore the role of OIP5 gene in renal cancer and the effect of Sorafenib on Raf1/OIP5 pathway.Methods Before and after up-regulation of OIP5 gene,cell proliferation and migration were detected with CCK-8 and cell migration assays,and cell invasion was tested with invasion assay.After down-regulation of Raf1 gene,the RNA and protein expression of OIP5 gene in786-O cells were determined.After 786-O cells were treated with 5μmol/L and 10μmol/L Sorafenib for 48 hours,the expression of Raf1 and OIP5 genes was tested.Results The proliferation and migration of 786-O cells were obviously enhanced after up-regulation of OIP5 gene.In addition,the invasive ability of 786-O cells was significantly decreased after down-regulation of OIP5 gene.After down-regulation of Raf1 gene in 786-O cells,the RNA and protein expression of OIP5 gene were dramatically decreased.After 786-O cells were treated with 5μmol/L and10μmol/L Sorafenib for 48 hours,the expression of Raf1 and OIP5 genes was obviously decreased.Conclusion OIP5 gene plays a oncogenil role in the progression of renal cancer.Sorafenib can inhibit the development of renal cancer through Raf1/OIP5 pathway.