甲醛暴露对胎鼠心肌组织基因表达的影响

Exposure of formaldehyde affects fatal rats' myocardial tissue gene expression

目的通过对胎鼠心肌组织基因表达谱的检测,探讨大鼠孕前及孕早期甲醛暴露对胎鼠心肌组织基因表达的影响。方法将性成熟的雌性SD大鼠随机分为实验组(甲醛吸入组)和对照组。甲醛吸入组母鼠在受孕前13 d给予甲醛吸入至怀孕第14天,对照组母鼠不进行甲醛吸入,两组母鼠喂养方式无差异。在SD大鼠怀孕第14天时取出胎鼠,对胎鼠心肌组织进行基因芯片检测分析。结果母鼠甲醛吸入组的胎鼠心肌样本与母鼠未吸入甲醛的胎鼠心肌样本相比,有25种与心脏发育相关基因的表达出现上调,且其上调倍数均大于3倍。结论雌性SD大鼠甲醛暴露对其胎鼠心肌中与心脏发育相关的基因表达有影响,为进一步进行先天性心脏病发病机制的研究提供了依据。

Objective To explore the association of formaldehyde exposure in pre-pregnant and early pregnancy female rats and their fatal rats＇ myocardial tissue gene expression. Methods SD female rats were randomly divided into experimental group and control group. Rats in experimental group exposure to formaldehyde inhalation from 13 days before conception to 14 days of pregnancy,and rats in control group was not exposed to formaldehyde. The feeding way was same in two groups. Fetal rats＇ myocardial tissues were prepared for microarray detection after 14 days of pregnancy. Results 25 genes were observed related with heart tissue development were up-regulated more than 3 times in experimental group than those expressions in control group. Moreover,those genes were involved in the transmission of 5 kinds of important signaling pathways involved in the development of heart tissue. Conclusion Early pregnancy formaldehyde inhalation exposure can significantly increase the expression of genes involved in the transmission of signal path of heart tissue development.