两面针内生真菌Y118菌株的抗HepG2肿瘤细胞研究

Study on anti HepG2 tumor cells of Y118 strains of Zanthxylumnitidum(Roxb.) DC endophytic fungus

目的:分离筛选两面针内生真菌Y118菌株及其代谢产物抗HepG2肿瘤细胞活性部位。方法:采用MTT比色法对两面针内生真菌Y118菌株及发酵代谢产物进行抗HepG2肿瘤细胞活性测定。结果:菌株Y118发酵后的菌丝体甲醇提取物,丙酮提取物和乙酸乙酯提取物浓度为80ug/ml时均对HepG2细胞有抑制作用。菌株的发酵液对HepG2细胞有明显的抑制作用。当浓度达到5%时,对HepG2细胞的抑制率达到43.94%;当浓度为10%时,对HepG2细胞的抑制率达到74.34%。结论:菌株Y118的甲醇提取物,丙酮提取物、乙酸乙酯提取物和发酵液均对HepG2细胞有抑制作用,其中发酵液的抑制作用较为明显。

Objective：To isolate and sift the Y118 strains of Zanthxylumnitidum（Roxb.）DC endophytic fungi and active sites of their metabolites against HepG2 tumor cells.Methods：MTT colorimetric assay was used to determine the activity of the Y118 strains of endophytic fungi and active sites of their metabolites against HepG2 tumor cells.Results：The methanol extract, acetone extract and ethyl acetate extract concentrations of Y118 strains of 80ug · ml^-1 after fermentation were inhibited by HepG2 cells.The fermentation liquid of the strains has obvious inhibitory effect on the HepG2 cells.When the concentration reaches 5%,the inhibition rate of HepG2 cells reaches 43.94%;When the concentration is 10%,the inhibition rate of HepG2 cells reaches 74.34%.Conclusion：The methanol extract, acetone extract,ethyl acetate extract concentrations of Y118 strains and fermentation liquid after fermentation have inhibitory effect on the HepG2 cells,and the inhibition of fermentation liquid is obvious.