椰子SSR分子标记筛选

Screening of SSR Markers for Coconut

[目的]筛选可用的SSR分子标记,加快椰子分子辅助育种进程。[方法]采用改良CTAB法提取椰子基因组DNA,以基因组DNA为模板,对36对SSR引物进行筛选,以条带清晰、多态性丰富为引物筛选原则。[结果]共筛选出7对具有多态性的引物,其中2对多态性较丰富,可用于后续SSR分子标记分析。[结论]该研究为开展椰子种质资源遗传多样性系统分析提供了SSR引物。

[Objective]To accelerate the molecular assisted breeding process of coconut by screening SSR markers.[Method]The genomic DNA was extracted by modified CTAB method,and 36 SSR primers were screened with genomic DNA as template.[Result]A total of 7 pairs of polymorphic primers were screened,of which more than 2 pairs of polymorphism were found,which could be used for subsequent SSR molecular marker analysis.[Conclusion]The study provides the SSR primers for analysis of coconut germplasm genetic diversity system.