黄芪注射液对小鼠急性胃黏膜损伤的保护作用及机制

The protective effect and mechanism of Astragali Radix injection on acute gastric mucosa injury in mouse models

目的研究黄芪注射液对小鼠急性胃黏膜损伤的保护作用及机制。方法将50只小鼠随机平均分为正常对照组、模型对照组(给予0.9%Na Cl注射液)、西咪替丁(25 mg/kg)组、黄芪注射液低浓度组(给予300 mg/ml黄芪注射液)及黄芪注射液高浓度组(给予600 mg/ml黄芪注射液),采用灌胃给药方式,1次/d,共14 d。在末次灌胃30 min后,正常对照组(给予0.9%Na Cl注射液)、模型对照组、黄芪注射液低浓度组和黄芪注射液高浓度组(给予0.2%吲哚美辛溶液)灌胃,4 h后观察每组小鼠的胃黏膜损伤程度,检测每组血清中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果黄芪注射液、西咪替丁等药物干预组和模型对照组的胃黏膜损伤指数与正常对照组相比均显著升高(P<0.01);黄芪注射液、西咪替丁等药物干预组小鼠胃黏膜损伤指数比模型对照组显著降低,差异有统计学意义(P<0.01);黄芪注射液高浓度组小鼠的胃黏膜损伤指数比黄芪注射液低浓度组和西咪替丁组显著降低,差异有统计学意义(P<0.01);模型对照组的SOD活性及MDA含量比正常对照组显著升高,差异有统计学意义(P<0.01);西咪替丁组、黄芪注射液低浓度组和黄芪注射液高浓度组中SOD活性、MDA含量比模型对照组均显著降低,差异有统计学意义(P<0.01);黄芪注射液高浓度组SOD活性比西咪替丁组显著降低,差异有统计学意义(P<0.01)。结论黄芪注射液对小鼠急性胃黏膜损伤具有保护作用,考虑与SOD活性、MDA含量降低有关。

Objective To study the protective effect and mechanism of Astragali Radix injection on acute gastrie mucosa injury in mouse models. Methods 50 mice were randomly divided into the normal control group, the model group（treated with 0. 9% NaCl injection） , the cimetidine group（treated with cimetidine 25 mg/kg） , the low Astragali Radix group（ injected with Astragali Radix injection 300 mg/ml） and the high Astragali Radix group（ treated with Astragali Radix injection 600 mg/ml）. Then, the drugs were infused into the stomach of mice once a day for 2 weeks. 30 minutes after the last medication, the normal control group was injected with 0.9% NaC1 while the model group, the low and high Astragali Radix groups were injected by gavage with 0. 2% indomethaein solution. 4 hours later, the gastric mucosa injury was observed and the serum superoxide dismutase（SOD） and methane diearboxylie aldehyde（MDA） were tested for each group. Results Compared with that in the normal eontrol group, the gastric mueosa injury in the drug intervention groups（ treated with Astragali Radix injection and eimetidine） was more severe （P 〈 0. 01 ）, however, the gastric mucosa injury in the drug intervention groups （treated with Astragali Radix injec- tion and cimetidine） was milder than that in the model group（P 〈 0. 01 ）. The gastric mueosa injury in high or low Astragali Radix injection group was milder than that in the cimetidine group（P 〈0. 01 ）. On the contrary, the levels of SOD and MDA in the model group were higher than those in the normal control group（P 〈 0. 01 ）. Compared with those in the model group, the levels of SOD and MDA in the drug intervention groups （ treated with Astragali Radix injection and cimetidine） were lower（ P 〈 0. 01 ）. The level of SOD in the high Astragali Radix injection group was sig- nificantly lower than that in the cimetidine group. Conclusion Astragali Radix injection has a protective effect on acute gastric mucosa injury of mice, whose mechanism may be related to reducing the levels of SOD and MDA.