基于“膜污染思维”的酶固定化方法及其应用

Enzyme immobilization inspired by “membrane fouling” mechanism and its application

将生物酶固定在膜面/内制备生物催化膜,可以增加酶的稳定性,实现酶重复使用以及在线移出产物以降低反馈抑制,在生化反应-分离耦合过程中具有得天独厚的优势.浓差极化和膜污染是膜分离过程中的瓶颈问题,但事实上膜污染机理与酶固定化策略具有共通点,因此建立基于"膜污染思维"的酶固定化方法,利用膜污染的形成机理/模型来调控和优化酶固定化过程,开发一系列的新型酶固定化方法,并成功应用于二氧化碳转化、水中微量污染物的去除和蛋白质水解.

Immobilizing enzymes in/on the filtration membranes can increase enzyme stability, achieve enzyme reuse and in-situ removal of product （relieve product inhibition）, and play an important role in biochemical reaction-separation integrated process. Concentration polarization and membrane fouling are the bottleneck problems in the membrane separation process, while actually there are some connections between membrane fouling mechanisms and enzyme immobilization strategies. And thus we proposed a novel enzyme immobilization method inspired by membrane fouling. Taking advantage of fouling formation mechanisms and mathematical models, enzyme immobilization process could be regulated and optimized, and a series of enzyme immobilization strategies were developed and successfully applied in CO2 bioconversion, micro-pollutant removal and protein hydrolysis.