急性颅脑损伤病人血浆H_2S和TNF-α相关性分析

Analysis of correlation of plasma levels of hydrogen sulfide and tumor necrosis factor-α with severity of traumatic brain injury

目的探讨急性颅脑损伤病人血浆硫化氢(H_2S)与肿瘤坏死因子-α(TNF-α)的相关性。方法 2015年3月至2016年10月收治急性颅脑损伤42例,其中轻型14例,中型13例,重型15例;以同期健康体检者30例作为对照组。采用亚甲基蓝法测定血浆H_2S水平,采用双抗体夹心酶联免疫吸附法检测血浆TNF-α的浓度。结果与对照组比较,颅脑损伤病人血浆H_2S水平显著降低(P<0.05),随颅脑损伤程度加重,血浆H_2S明显降低(P<0.05)。颅脑损伤血浆TNF-α水平变化与H_2S相反。轻型颅脑损伤病人血浆H_2S与TNF-α水平无明显相关性(P>0.05)。中型、重型颅脑损伤血浆H_2S与TNF-α呈明显负相关(r分别为-0.46、-0.52;P<0.05)。结论本研究结果提示颅脑损伤后内源性H_2S生成减少可能是导致炎症因子生成增加的原因之一。

Objective To explore the correlation of plasma levels of hydrogen sulfide （H2S） and tumor necrosis factor-α （TNF-α） with severity of traumatic brain injury （TBI）. Methods The plasma levels of H2S and TNF-α were determined respectively by methylene blue method and double antibody sandwich ELISA in 14 patients with mile TBI, 13 patients with moderate TBI, 15 patients with severe TBI and 30 healthy subjects. Results The plasma level of H2S was significantly lower in the patients with severe TBI than that in the patients with moderate TBI （P〈0.05）, which was significantly lower than that in the patients with mild TBI （P〈0.05）, which was significantly lower than that in the healthy subjects （P〈0.05）. The plasma level of TNF-α was significantly higher in the patients with severe TBI than that in the patients with moderate TBI （P〈0.05）, which was significantly higher than that in the patients with mild TBI （P〈0.05）, which was significantly higher than that in the healthy subjects （P〈0.05）. The plasma TNF-α levels were negatively related to the plasma H2S levels in the patients with moderate or severe TBI （P〈0.05）. Conclusion It is suggested that the changes in the plasma levels of H2S and TNF-α may play an important role in the pathophysiologic process after TBI.